FastQCFastQC Report
Wed 28 Jun 2023
SRR1585518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27182328
Total Bases761.4 Mbp
Sequences flagged as poor quality0
Sequence length21-42
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGAGCGACGCTCAGACAGG3540341.3024417923291927No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT1339660.49284226133979403No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG1246920.45872450659855185No Hit
GGAGAGCGACGCTCAGACAGGC1091100.40140049814717854No Hit
CCTCTAGCATCACCAGTATTAGAGGC978690.3600464242797747No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT787900.2898574397306956No Hit
GCCGGCCGAGGGTGGGATCCCGAGGCC774160.284802685038603No Hit
AAAGCACCCAACTTACACTTAGGAGATT662150.24359576560182777No Hit
ACCTCTAGCATCACCAGTATTAGAGGC506230.18623496854279736No Hit
CTCTAGCATCACCAGTATTAGAGGC462580.1701767413004508No Hit
AATACAAGCTTGGGCTGCAGGTCGAC431510.15874652090137387No Hit
GGAGCGCGACGCTCAGACAGG354460.13040089870153873No Hit
GGAGAGATCCTTCCGCAGGTTC352850.1298086021182586No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC314270.11561555728412959No Hit
GGTCGGTTTCTATCTACTTCAAATTCCTC305020.1122126110758431No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC301830.11103905449157997No Hit
CAAAGCACCCAACTTACACTTAGGAGATT297180.10932838423552242No Hit
GGTCGGTTTCTATCTACTTCAAATTCCT295850.10883909575368231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)