FastQCFastQC Report
Wed 28 Jun 2023
SRR1585519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25721545
Total Bases727.3 Mbp
Sequences flagged as poor quality0
Sequence length21-42
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGAGCGACGCTCAGACAGG2913691.1327818760498252No Hit
GGAGAGCGACGCTCAGACAGGC1053120.40943108199760164No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG867300.3371881432472272No Hit
CCTCTAGCATCACCAGTATTAGAGGC515650.20047396064272188No Hit
GATCAAAAGGGACAAGCATCAAGCACGCAAC492170.1913454265674943No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC375500.14598656495945325No Hit
GGAGAGATCCTTCCGCAGGTTC354720.13790773454704994No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC344810.1340549333253504No Hit
ACCTCTAGCATCACCAGTATTAGAGGC325210.12643486229151477No Hit
GGTCGGTTTCTATCTACTTCAAATTCCTC291050.1131541670611155No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT276260.10740412366364463No Hit
CTCTAGCATCACCAGTATTAGAGGC266970.10379236550526028No Hit
AATACAAGCTTGGGCTGCAGGTCGAC264490.10282819325199945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)