FastQCFastQC Report
Wed 28 Jun 2023
SRR1585521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13545825
Total Bases385.3 Mbp
Sequences flagged as poor quality0
Sequence length21-48
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCAATTTTTGACAGTCTCTACGGAGACTG1915661.4142069604472225No Hit
GGCAATTTTTGACAGTCTCTACGGAGACTG1049890.7750653799233344No Hit
AGCTTGGGCTGCAGGTCGACCCGTGC741670.5475266364359499No Hit
ACCTCTAGCATCACCAGTATTAGAGGC496460.36650407044236877No Hit
AAACGCTGCCGCCCGGCGATGCGGCGGCG450840.332825796878374No Hit
CCTCTAGCATCACCAGTATTAGAGGC432170.31904295234878643No Hit
TGGCACTTTTTGACAGTCTCTACGGAGACTG408120.3012884043607532No Hit
AAGGCCTACTTCACAAAGCGCCTTCC401320.29626840742442784No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT393940.2908202342788276No Hit
TGACAGTCTCTACGGAGACTG271520.20044552472809887No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG255270.18844920851996833No Hit
ACCTCTAGCATCACCAGTATTAGAGGCA252210.18619020989862192No Hit
GATCAAAAGGGACAAGCATCAAGCACGCAAC250130.1846546814239812No Hit
TTGACAGTCTCTACGGAGACTG222050.1639250470163316No Hit
AAACGCTGCCGCCCGGCGATGCGGCGGCGTT211130.15586352252446786No Hit
GGCAATTTTTGCCAGTCTCTACGGAGACTG195340.14420679434438288No Hit
TGCTAAGACTTCACCAGTCAAAGCGA192210.14189611928398602No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCC165530.12220001365734462No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAAC165430.12212619017298688No Hit
AAAGCACCCAACTTACACTTAGGAGATT164330.12131413184505188No Hit
GCAATTTTTGACAGTCTCTACGGAGACTG146920.1084614632183717No Hit
ACTGGCAATTTTTGACAGTCTCTACGGAGACTG146120.1078708753435099No Hit
AAACGCTGCCGCCCGGCGATGCGGCGGCGTTA143940.10626152338451145No Hit
CCTACTTCACAAAGCGCCTTCC141180.10422399521623821No Hit

[OK]Adapter Content

Adapter graph