FastQCFastQC Report
Wed 28 Jun 2023
SRR1585525.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585525.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18831560
Total Bases523.6 Mbp
Sequences flagged as poor quality0
Sequence length21-48
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCTGGGAATACCGGGTGCTGTAGGCTT701000.37224743993593734No Hit
CCTCTAGCATCACCAGTATTAGAGGC489000.2596704680865526No Hit
ACTATGCCGACCTGCGATGAGGCGGCT461950.24530628370671362No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT422530.22437333922415348No Hit
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCG417770.2218456675920635No Hit
CTCTAGCATCACCAGTATTAGAGGC402860.21392810792095823No Hit
AGCTTGGGCTGCAGGTCGACCCG311540.16543504627338362No Hit
TGCTAAGACTTCACCAGTCAAAGCGAA285770.15175057191225794No Hit
ACCTCTAGCATCACCAGTATTAGAGGC270170.14346660605919No Hit
AAACGCTGCCGCCCGGCGATGCGGCGGCG264850.14064156129391298No Hit
ACTATGCCGACCTGCGATGCGGCGGCG231470.12291599846215608No Hit
AAACGCTGCCGACCGGCGCTGCGGCGGCG210630.11184946971998071No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG202840.10771279702796795No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC201660.10708618935446665No Hit
ACTATTCCGACCCGAGATGAGGAGGAC200430.10643303050835937No Hit
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCGC190910.10137768724417945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)