FastQCFastQC Report
Wed 28 Jun 2023
SRR1585527.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585527.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12147124
Total Bases354.7 Mbp
Sequences flagged as poor quality0
Sequence length21-48
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCG1291641.0633298877989557No Hit
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCGC1109150.9130967955871695No Hit
TTGGGGGGCCGGGCCCCCCCTCCCCCGGCG366390.301626953013734No Hit
TTGGGGGGCCGGGCCCCCCCTCCCCCGGCGC337790.2780822851565523No Hit
TGCTAAGACTTCACCAGTCAAAGCGAA319600.2631075471033308No Hit
CCTCTAGCATCACCAGTATTAGAGGC228830.18838204006149933No Hit
ACCTCTAGCATCACCAGTATTAGAGGC225130.1853360515624933No Hit
TTGGGGGGCCGGGCCACCCCTCCCCCGGCGC205970.16956277057845132No Hit
TTGGGGGGCCGGGCCACCCCTCCCCCGGCG190600.15690956970555334No Hit
GGGGGGGGGCCACGCGCGCGTCCCCC187120.15404469403621796No Hit
TCGACTGCATCCTTTGTGGTAGTGGGGG176380.14520309498775183No Hit
TTGGGGGGCCGGGCCCCCCCTCCCACGGCG161470.13292858457689244No Hit
TTGGGGGGCCGGGCCCCCCCTCCCACGGCGC139530.11486669601792161No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC135670.11168898909733696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)