FastQCFastQC Report
Wed 28 Jun 2023
SRR1585529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18638983
Total Bases525.8 Mbp
Sequences flagged as poor quality0
Sequence length21-42
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGAAGGTGGCTCGGGGGGC1301320.6981711394875997No Hit
GGAGAGCGACGCTCAGACAGGC1115220.5983266361689369No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG738660.3962984461115716No Hit
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCGC698390.3746931900737288No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT653240.35046976543730957No Hit
GATCAAAAGGGACAAGCATCAAGCACGCAAC650420.34895680735370593No Hit
CGGGGGAAGGTGGCTCGGGGGG559910.3003972909895352No Hit
GGAGAGCGACGCTCAGACAGG519250.27858279606778974No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC502800.26975720724676877No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC425750.22841911492703224No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT292740.15705792531706264No Hit
GGTCGGTTTCTATCTACTTCAAATTCCT266710.14309257109145923No Hit
GGAGAGCGACGCTCAGACAGGCG247020.1325286900041703No Hit
AATACAAGCTTGGGCTGCAGGTCGAC242470.130087569691973No Hit
GGCCCGGCGGGGGAAGGTGGCTCGGGGGGC221630.11890670215215068No Hit
GGTCGGTTTCTATCTACTTCAAATTCCTC214680.11517795793901417No Hit
CCTCTAGCATCACCAGTATTAGAGGC211150.11328407778471604No Hit
AGCTTGGGCTGCAGGTCGACCCG189700.10177593917007167No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCC189570.10170619287543747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)