FastQCFastQC Report
Wed 28 Jun 2023
SRR1585530_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585530_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13133900
Total Bases377.2 Mbp
Sequences flagged as poor quality0
Sequence length21-42
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTAGCATCACCAGTATTAGAGGC525210.39988883728367053No Hit
ACCTCTAGCATCACCAGTATTAGAGGC444190.3382011436054789No Hit
TGGGGGGGCCGGGCCCCCCCTCCACCGGCG440550.3354296895819216No Hit
CTCTAGCATCACCAGTATTAGAGGC388840.2960582919011109No Hit
TGCTAAGACTTCACCAGTCAAAGCGAA341520.2600293895948652No Hit
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCG329130.25059578647621805No Hit
TGGGGGGGCCGGGCCCCCCCTCCACCGGCGC255740.1947174868089448No Hit
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCGC216250.1646502562072195No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG213280.162388932457229No Hit
GCTAAGACTTCACCAGTCAAAGCGAA185060.14090254988997936No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC179930.13699662704908672No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT178780.1361210303108749No Hit
TGCTAAGACTTCACCAGTCAAAGCGA164530.12527124464172867No Hit
GGAGAGCGACGCTCAGACAGGC163970.1248448670996429No Hit
TTGGGGGGCCGGGCCACCCCTCCCCCGGCG161300.1228119598900555No Hit
GATCAAAAGGGACAAGCATCAAGCACGCAAC140500.10697507975544203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)