FastQCFastQC Report
Wed 28 Jun 2023
SRR1585531.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585531.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19502450
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length21-48
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTAGCATCACCAGTATTAGAGGC759930.38965873518455374No Hit
CTCTAGCATCACCAGTATTAGAGGC645300.33088150463146937No Hit
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCG637580.32692302761960673No Hit
AGCTTGGGCTGCAGGTCGACCCG593600.30437201479814074No Hit
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCGC429150.22004927586021245No Hit
ACCTCTAGCATCACCAGTATTAGAGGC418270.2144704896051522No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT347270.178064807242167No Hit
ACTATGCCGACCTGCGATGAGGCGGCT318000.16305643649900398No Hit
TCGACTGCATCCTTTGTGGTAGTGGGGG313630.1608156923873667No Hit
TTTTTGACAGTCTCTACGGAGACTG287910.14762760576235293No Hit
TGCTAAGACTTCACCAGTCAAAGCGAA282480.144843340195719No Hit
ATTTTTGACAGTCTCTACGGAGACTG224990.11536499260349342No Hit
AAACGCTGCCGCCCGGCGATGCGGCGGCG209130.10723268102212798No Hit
TGCTAAGACTTCACCAGTCAAAGCGA202870.10402282790110985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)