FastQCFastQC Report
Wed 28 Jun 2023
SRR1585536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18592271
Total Bases528.4 Mbp
Sequences flagged as poor quality0
Sequence length21-42
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTAGCATCACCAGTATTAGAGGC486300.26156030105198014No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC409060.2200161561758647No Hit
TGGGGGGGCCGGGCCCCCCCTCCACCGGCG350830.18869669014613652No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT341770.18382369749236122No Hit
ACCTCTAGCATCACCAGTATTAGAGGC341380.18361393290792719No Hit
CTCTAGCATCACCAGTATTAGAGGC312550.1681074893970726No Hit
TGCTAAGACTTCACCAGTCAAAGCGAA249210.13403956945334972No Hit
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCG225470.12127082269831373No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG222040.1194259700711118No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC203970.10970687765900142No Hit
TGGGGGGGCCGGGCCCCCCCTCCACCGGCGC196330.10559764323572951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)