FastQCFastQC Report
Wed 28 Jun 2023
SRR1585539.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585539.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31202186
Total Bases894.9 Mbp
Sequences flagged as poor quality0
Sequence length21-48
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTGGGCTGCAGGTCGACCCGTGC7114592.280157550499827No Hit
AATACAAGCTTGGGCTGCAGGTCGAC789590.25305598780803373No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG615280.1971913121728074No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAA516660.16558455231309754No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTC414240.13275992906394443No Hit
AAAGCACCCAACTTACACTTAGGAGATT389510.12483420232159376No Hit
GATCAAAAGGGACAAGCATCAAGCACGCAGC387560.12420924610858995No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAA385940.12369005171624833No Hit
GGAGAGCGACGCTCAGACAGGCGT353030.11314271378293815No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAA349680.11206907105803421No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAA335900.10765271381947407No Hit
TGGTCCGATGGTAGTGGGTTATCAGAA322530.10336775763082753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)