FastQCFastQC Report
Wed 28 Jun 2023
SRR1585542.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585542.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27119206
Total Bases781.6 Mbp
Sequences flagged as poor quality0
Sequence length21-48
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTGGGCTGCAGGTCGACCCGTGC6050282.230994521004782No Hit
GGAGAGCGACGCTCAGACAGG475920.17549186358922161No Hit
GGAGAGCGACGCTCAGACAGGC448500.16538094810002918No Hit
AATACAAGCTTGGGCTGCAGGTCGAC403870.14892397660904969No Hit
TCGACTGCCTAATTTGTGGTCGTGGGGG319860.117945931012877No Hit
ACCTCTAGCATCACCAGTATTAGAGGC286820.10576268346499526No Hit
CCTCTAGCATCACCAGTATTAGAGGC285650.1053312549047343No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTC275370.10154058345218514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)