FastQCFastQC Report
Wed 28 Jun 2023
SRR1585543.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585543.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15566409
Total Bases441.3 Mbp
Sequences flagged as poor quality0
Sequence length21-48
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTGGGCTGCAGGTCGACCCGTGC2102181.3504591842601592No Hit
CCTCTAGCATCACCAGTATTAGAGGC864950.5556515956891535No Hit
ACCTCTAGCATCACCAGTATTAGAGGC653920.420084041219783No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT528890.3397636539037359No Hit
CTCTAGCATCACCAGTATTAGAGGC462380.2970370366087644No Hit
GGTACACGGAGTCAAGACCCGCAACGCGG416020.2672549590596007No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC397700.255486027638102No Hit
GGTACACGGAGTCAAGACCCGCAACGCGT298130.19152137143512032No Hit
TGTACACGGAGTCAAGACCCGCAACGCGG295980.19014019225628725No Hit
TGGTCCGATGGTAGTGGGTTATCAGAA291900.1875191638611063No Hit
TGCTAAGACTTCACCAGTCAAAGCGAA280090.17993231451133013No Hit
GGTACACGGAGTCGACCCGCAACGCGG276200.17743334381102283No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC269200.17293648136831044No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAA261860.16822119989266632No Hit
GGTACACGGAGTCGACCCGCAACGCGT215250.13827852011340575No Hit
TGTACACGGAGTCGACCCGCAACGCGG214510.13780313751231899No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAA205470.13199576087201614No Hit
TGCTAAGACTTCACCAGTCAAAGCGA182390.11716896298947305No Hit
AACGCTGCCGACCGGCGCTGCGGCGGCG176570.11343014307281789No Hit
CGTACACGGAGTCAAGACCCGCAACGCGG173820.11166351854175231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)