FastQCFastQC Report
Wed 28 Jun 2023
SRR1585545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28497536
Total Bases781.9 Mbp
Sequences flagged as poor quality0
Sequence length21-42
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATGCCGACCTGCGATGAGGCGGCT4288231.5047722020598553No Hit
ACTATGCCGACCTGCGATGCGGCGGCG2038740.7154092199409802No Hit
ACGATGCCGACCTGCGATGAGGCGGCT1525810.5354182200173376No Hit
ACTATGCCGACCTGCGATGCGGCGGCT1136700.398876590593657No Hit
GGAGAGCGACGCTCAGACAGG1088440.3819417931430984No Hit
AACTATTCAGACCGGCGATGCGGCGGCT961280.3373203914892852No Hit
GGAGAGCGACGCTCAGACAGGC941950.3305373489132534No Hit
AAACGATTCCGACCGGCGATGCGGCGTCG896260.3145043838176044No Hit
ACGATGCCGACCTGCGATGCGGCGGCT793490.2784416168471548No Hit
GGGACAAGCATCAAGCACGCAAC641940.22526158050997813No Hit
AAACGATTACGACCGGAGATGCGGCGTCG606530.21283594483396737No Hit
ACTATGCCGACCTGCGATGAGGCGGCG586120.20567392212435487No Hit
ACGATGCCGACCTGCGATGAGGCGGCG575540.2019613204453887No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG517300.18152446583451987No Hit
CCTCTAGCATCACCAGTATTAGAGGC505670.17744341124790577No Hit
GGACAAGCATCAAGCACGCAAC483170.16954799179830846No Hit
AAACGATTCCGACCGGAGATGCGGCGTCG440730.15465547617871245No Hit
ACTATGCCGACCGGCGATGAGGCGGCT434310.152402649829094No Hit
TCTACTTCATAATTTGTGGTAGTGGGTT402120.1411069364032034No Hit
TCTACTTCATAATTTGTGGTAGTGGGT386990.1357977054577631No Hit
ACTATGCCGACCGGCGATGAGGCGGCG373490.13106045378800468No Hit
ACGATGCCGACCGGCGATGAGGCGGCT370550.13002878564659065No Hit
ACTATGCCGACCTGCGATGAGGCGGCC323850.11364140394453752No Hit
CTCTAGCATCACCAGTATTAGAGGC315060.11055692674622818No Hit
AACGATTCAGACCGGCGATGCGGCGGCT304880.10698468807969924No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT300340.10539156788853606No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)