FastQCFastQC Report
Wed 28 Jun 2023
SRR1585548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30321530
Total Bases848.4 Mbp
Sequences flagged as poor quality0
Sequence length21-42
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATACAAGCTTGGGCTGCAGGTCGACCCG1394070.4597624196404337No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT992230.3272361256176717No Hit
AGCTTGGGCTGCAGGTCGACCCG855080.28200423923199125No Hit
GGAGAGCGACGCTCAGACAGGC765330.25240480938791676No Hit
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCGC751700.24790965363555204No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC650830.21464286267876323No Hit
GGTACACGGAGTCAAGACCCGCAACGCGG598130.19726247323271617No Hit
GGAGAGCGACGCTCAGACAGG542450.17889928377624742No Hit
AATACAAGCTTGGGCTGCAGGTCGAC505550.16672971317740232No Hit
CCTCTAGCATCACCAGTATTAGAGGC498410.164374950736325No Hit
GGTACACGGAGTCAAGACCCGCAACGCGT487770.16086589298099405No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT483370.15941477887164665No Hit
GGTCGGTTTCTATCTACTTCAAATTCCT456180.15044755327320225No Hit
GGTACACGGAGTCGACCCGCAACGCGG454460.1498802995759119No Hit
TGTACACGGAGTCAAGACCCGCAACGCGG433450.14295122970377816No Hit
GGTACACGGAGTCGACCCGCAACGCGT428180.14121319075917343No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC415140.13691261621692571No Hit
TGTACACGGAGTCGACCCGCAACGCGG395100.13030345104617083No Hit
AATACAAGCTTGGGCTGCAGGTCG382760.12623373556677384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)