FastQCFastQC Report
Thu 29 Jun 2023
SRR1585892.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585892.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18086005
Total Bases904.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA14384587.953431396264681RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC10733975.934959102355662RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG7489334.1409531845202965RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC6206963.4319132389933547No Hit
TCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCC2527641.3975667926664845RNA PCR Primer, Index 1 (100% over 27bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC2098231.160140119390656No Hit
TCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC2029521.1221494188462295RNA PCR Primer, Index 1 (100% over 26bp)
ACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCA2011951.1124347250816309RNA PCR Primer, Index 1 (100% over 28bp)
TGTGGCGTCGCTGAACCATAGTGGAATTCTCGGGTGCCAAGGAACTCCAG1798030.9941554257007006RNA PCR Primer, Index 1 (100% over 29bp)
TCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCA1499260.8289613986062705RNA PCR Primer, Index 1 (100% over 28bp)
GTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1446140.7995906226941771RNA PCR Primer, Index 1 (100% over 31bp)
ACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1395480.7715800144918681RNA PCR Primer, Index 1 (100% over 29bp)
TCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCA1319400.7295143399551199RNA PCR Primer, Index 1 (100% over 28bp)
CGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1305810.7220002427291157RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGCCA1273090.7039089063615763No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCA1249400.6908103807336115No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG1231050.6806644142805445Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCC1150770.6362765021905059No Hit
GTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1138330.6293982557231406RNA PCR Primer, Index 1 (100% over 30bp)
TTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCCA1110930.6142484202564359No Hit
TGTGGCGTCGCTGAACCATAGCTGGAATTCTCGGGTGCCAAGGAACTCCA687540.38015028747365714RNA PCR Primer, Index 1 (100% over 28bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCGGGTGCCAAG601900.33279875793465724No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGC601100.3323564269721257No Hit
TTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCC559320.3092556924539167RNA PCR Primer, Index 1 (100% over 27bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGC515960.28528135428470797No Hit
ACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT510790.28242279043934804RNA PCR Primer, Index 1 (100% over 30bp)
TCGGTGCCCGAGTTGTAATTTTGGAATTCTCGGGTGCCAAGGAACTCCAG493270.27273574235990755RNA PCR Primer, Index 1 (100% over 29bp)
GGCGCCAGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA460630.25468863908862127RNA PCR Primer, Index 1 (100% over 28bp)
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGGA456820.25258203787956485RNA PCR Primer, Index 1 (100% over 22bp)
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTC449550.24856235525755965RNA PCR Primer, Index 1 (100% over 26bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA449540.248556826120528No Hit
TTGTGGCGTCGCTGAACCATAGTGGAATTCTCGGGTGCCAAGGAACTCCA441560.244144574769276RNA PCR Primer, Index 1 (100% over 28bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG431300.2384716801748092Illumina Small RNA Adapter 2 (100% over 21bp)
CCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCC424970.2349717364337785RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGGTTTATCTCGTATGC424530.23472845440438614RNA PCR Primer, Index 14 (97% over 37bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAGG407480.225301275765433Illumina Small RNA Adapter 2 (100% over 21bp)
TACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA406370.2246875415549205RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAA401220.2218400359836238No Hit
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA361290.19976219181626897RNA PCR Primer, Index 1 (100% over 23bp)
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGGA358500.19821956258444026RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAACT334380.18488328406411478RNA PCR Primer, Index 1 (100% over 25bp)
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC333110.18418108366109598No Hit
CCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC321570.17780045952657872RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTCGAATATATTAGCATGGAATAATAGATGGAATTCTCGGGTGCCAA308470.17055729001512496No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAGGA306730.16959522017161888RNA PCR Primer, Index 1 (100% over 22bp)
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAA303050.16756049774397386No Hit
TACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC298740.16517743968333526RNA PCR Primer, Index 1 (100% over 27bp)
GTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC289480.16005745879203284RNA PCR Primer, Index 1 (100% over 31bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG279940.1547826620638444No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGA279280.1544177390197559RNA PCR Primer, Index 1 (100% over 22bp)
CACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAACTC261270.1444597632257649RNA PCR Primer, Index 1 (100% over 26bp)
ATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACTCC257560.14240845338702493RNA PCR Primer, Index 1 (100% over 27bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG245760.13588407168968492No Hit
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGAA244570.1352261043829193RNA PCR Primer, Index 1 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACTC243790.13479483169445106RNA PCR Primer, Index 1 (100% over 26bp)
TTCGGTGCCCGAGTTGTAATTTTGGAATTCTCGGGTGCCAAGGAACTCCA236850.13095761059449004RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCA235640.1302885850136611RNA PCR Primer, Index 1 (100% over 28bp)
AGCGGAGAATTTGGACAAACTTTGGAATTCTCGGGTGCCAAGGAACTCCA233850.12929886948499683RNA PCR Primer, Index 1 (100% over 28bp)
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG231420.12795528918630733No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAGG228970.12660065061355452Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTGCCA226830.12541741528878267No Hit
GGCGCCAGTGAAATACCACTACCTGGAATTCTCGGGTGCCAAGGAACTCC224510.12413465549744125RNA PCR Primer, Index 1 (100% over 27bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAA222890.12323893529831492No Hit
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT218670.12090563947096111RNA PCR Primer, Index 1 (100% over 25bp)
TTTCGGTGCCCGAGTTGTAATTTTGGAATTCTCGGGTGCCAAGGAACTCC218430.12077294018220165RNA PCR Primer, Index 1 (100% over 27bp)
GGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT215790.11931324800584762RNA PCR Primer, Index 1 (100% over 30bp)
GGCGCCAGTGAAATACCACTACCTTGGAATTCTCGGGTGCCAAGGAACTC209500.11583542081294348RNA PCR Primer, Index 1 (100% over 26bp)
TACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG209350.11575248375746883RNA PCR Primer, Index 1 (100% over 29bp)
GTGGCGTCGCTGAACCATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT207660.11481805959912096RNA PCR Primer, Index 1 (100% over 30bp)
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCGGGTGCCAAGG205360.11354635808184285Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGCGTCGCTGAACCATAGCATGGAATTCTCGGGTGCCAAGGAACTCC203190.11234653534597608RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCTAGCATTCAAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT202220.1118102090539066RNA PCR Primer, Index 1 (100% over 30bp)
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA201390.11135129068028013RNA PCR Primer, Index 1 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGTGC192320.10633636339257896No Hit
CGGTGCCCGAGTTGTAATTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT187640.10374872726176952RNA PCR Primer, Index 1 (100% over 30bp)
GTGGCGTCGCTGAACCATAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG186950.10336721680658609RNA PCR Primer, Index 1 (100% over 29bp)
TGCCAAGGACGTTTTCATTAATCAAGAACGAATGGAATTCTCGGGTGCCA184830.10219503975587754No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)