FastQCFastQC Report
Thu 29 Jun 2023
SRR1585893.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585893.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23442151
Total Bases937.6 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC2550941.088185124308772No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTC1755810.7489969670445344No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG1438580.6136723545548358No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGG1097020.4679690016500619No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTC913630.3897381259936428No Hit
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGC900220.3840176611779354No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCG823980.3514950483852783No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAAT769820.3283913664748598No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGG743670.31723624679322304No Hit
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGG623710.26606346832251015No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGG605980.25850016920375607No Hit
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGG566620.2417099011093308No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC558200.23811808054644817No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCG510030.21756962490344847No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGG502090.21418256370757102No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTC480820.2051091642571537No Hit
TCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTG475370.20278429227761566No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG470120.2005447367009964No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTC460380.19638982787884954No Hit
ACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTG450010.1919661723875083No Hit
TCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGC430250.18353691177912812No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGG418680.17860135787027392No Hit
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTG402580.1717333874353083No Hit
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGG402240.1715883495503463No Hit
TCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGG392690.16751449131097226No Hit
TTTACCCAATCCTAATTCAGGGAGGTAGTGGAATTCTCGG389000.1659404036771199No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGTTA382210.16304391179802571RNA PCR Primer, Index 29 (97% over 38bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATT365370.1558602706722604No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATT340690.14533222655207706No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCT324180.13828935749112783No Hit
TCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCC320220.13660009271333506No Hit
TCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGG316030.1348127140721856No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGG315610.1346335496260561No Hit
TTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGT314070.1339766133235811No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGG299450.1277399842702148No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCT292110.12460887228309381No Hit
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGT282510.12051368494299008No Hit
AGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGT277380.11832531920812216No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTC275490.11751907920053922No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTC275130.11736550967528533No Hit
TGGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGG274350.11703277570390193No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCATGGAATTCT269470.11495105547268251No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCG254060.10837742662778685No Hit
TTTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTG252940.1078996547714414No Hit
TGGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCG251700.10737069307334467No Hit
ATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTG243180.10373621430900262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)