FastQCFastQC Report
Thu 29 Jun 2023
SRR1585896.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585896.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18974196
Total Bases948.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA14619257.704806042901634RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG10440855.502657398500574RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC8796884.636233334998753RNA PCR Primer, Index 1 (100% over 27bp)
TCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCA2426901.2790528779190433RNA PCR Primer, Index 1 (100% over 28bp)
TCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCC2357431.242439995876505RNA PCR Primer, Index 1 (100% over 27bp)
TGTGGCGTCGCTGAACCATAGTGGAATTCTCGGGTGCCAAGGAACTCCAG2296901.2105387759249457RNA PCR Primer, Index 1 (100% over 29bp)
GTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2135371.12540736903951RNA PCR Primer, Index 1 (100% over 31bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG2058831.0850683739116007Illumina Small RNA Adapter 2 (100% over 21bp)
ACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCA2015001.061968580908514RNA PCR Primer, Index 1 (100% over 28bp)
CGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1848820.9743864772979051RNA PCR Primer, Index 1 (100% over 29bp)
ACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1719400.9061780536050118RNA PCR Primer, Index 1 (100% over 29bp)
GTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1650860.8700553109075082RNA PCR Primer, Index 1 (100% over 30bp)
TCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCA1641770.8652645940834595RNA PCR Primer, Index 1 (100% over 28bp)
TCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC1559550.8219320597299616RNA PCR Primer, Index 1 (100% over 26bp)
TCGGTGCCCGAGTTGTAATTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1052240.5545636821713026RNA PCR Primer, Index 1 (100% over 29bp)
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGGA926060.4880628407127237RNA PCR Primer, Index 1 (100% over 22bp)
TTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCC767290.40438604091577846RNA PCR Primer, Index 1 (100% over 27bp)
ACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT764980.40316859802649874RNA PCR Primer, Index 1 (100% over 30bp)
GGCGCCAGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA719760.37933623116362875RNA PCR Primer, Index 1 (100% over 28bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCGGGTGCCAAG684690.3608532345718364No Hit
TGTGGCGTCGCTGAACCATAGCTGGAATTCTCGGGTGCCAAGGAACTCCA682240.3595620072650246RNA PCR Primer, Index 1 (100% over 28bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG622450.32805079066327764Illumina Small RNA Adapter 2 (100% over 21bp)
TTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCCA621930.32777673425530124No Hit
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTC621450.3275237591094769RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC587150.30944657681411114No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCTGAGATCTCGTATGC576480.3038231501350571RNA PCR Primer, Index 25 (97% over 38bp)
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG484200.25518867835032377No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGA468530.24693009390226603RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGCCA460300.2425926242144858No Hit
TTCGGTGCCCGAGTTGTAATTTTGGAATTCTCGGGTGCCAAGGAACTCCA422540.22269191274296943RNA PCR Primer, Index 1 (100% over 28bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAGG415980.21923458575003651Illumina Small RNA Adapter 2 (100% over 21bp)
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA414910.21867066198746973RNA PCR Primer, Index 1 (100% over 23bp)
TACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA406840.21441751734829767RNA PCR Primer, Index 1 (100% over 28bp)
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGAA399460.21052802448124813RNA PCR Primer, Index 1 (100% over 23bp)
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGGA396700.20907341739275806RNA PCR Primer, Index 1 (100% over 22bp)
TTGTGGCGTCGCTGAACCATAGTGGAATTCTCGGGTGCCAAGGAACTCCA396260.20884152350908572RNA PCR Primer, Index 1 (100% over 28bp)
AGCGGAGAATTTGGACAAACTTTGGAATTCTCGGGTGCCAAGGAACTCCA392690.206960020862017RNA PCR Primer, Index 1 (100% over 28bp)
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCGGGTGCCAAGG384050.20240646823717853Illumina Small RNA Adapter 2 (100% over 21bp)
TTTCGGTGCCCGAGTTGTAATTTTGGAATTCTCGGGTGCCAAGGAACTCC364040.1918605668456255RNA PCR Primer, Index 1 (100% over 27bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCA357580.18845594300807264No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG354820.18700133591958257No Hit
GTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC330630.1742524426331424RNA PCR Primer, Index 1 (100% over 31bp)
AGCGGAGAATTTGGACAAACTTGGAATTCTCGGGTGCCAAGGAACTCCAG323120.17029443566409877RNA PCR Primer, Index 1 (100% over 29bp)
CGGTGCCCGAGTTGTAATTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT316960.16704792129268614RNA PCR Primer, Index 1 (100% over 30bp)
GTGGCGTCGCTGAACCATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT311800.16432843847507425RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCC302270.15930582776735308RNA PCR Primer, Index 1 (100% over 27bp)
AGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG300030.1581252770868394RNA PCR Primer, Index 1 (100% over 29bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG296630.15633336980391688No Hit
TACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG288100.15183779064999645RNA PCR Primer, Index 1 (100% over 29bp)
TCGGTGCCCGAGTTGTAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC286210.150841701013313RNA PCR Primer, Index 1 (100% over 31bp)
CCTGATTCAAGCTCAGGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC283390.1493554720315949RNA PCR Primer, Index 1 (100% over 31bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAA270150.14237757425927297No Hit
GTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA269690.1421351397445246RNA PCR Primer, Index 1 (100% over 32bp)
GGCGCCAGTGAAATACCACTACCTGGAATTCTCGGGTGCCAAGGAACTCC252690.13317560332991182RNA PCR Primer, Index 1 (100% over 27bp)
TACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC252300.13297006102392955RNA PCR Primer, Index 1 (100% over 27bp)
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT249380.13143112888683137RNA PCR Primer, Index 1 (100% over 25bp)
CCTGCGGAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA246440.12988165611865715RNA PCR Primer, Index 1 (100% over 32bp)
GGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT245380.12932300267162836RNA PCR Primer, Index 1 (100% over 30bp)
GTGGCGTCGCTGAACCATAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG236850.12482742351770794RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCA229110.12074819929129013RNA PCR Primer, Index 1 (100% over 28bp)
GGCGCCAGTGAAATACCACTACCTTGGAATTCTCGGGTGCCAAGGAACTC227980.12015265363549527RNA PCR Primer, Index 1 (100% over 26bp)
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAAC221280.11662154222503024RNA PCR Primer, Index 1 (100% over 24bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA220520.11622099824414169RNA PCR Primer, Index 1 (100% over 22bp)
GCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGAA220150.1160259965692354RNA PCR Primer, Index 1 (100% over 23bp)
TTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCC219140.11549369469989663No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCAAGG215540.11359638110621394Illumina Small RNA Adapter 2 (100% over 21bp)
CTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC214250.11291651040181096RNA PCR Primer, Index 1 (100% over 33bp)
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA214100.11283745566874086RNA PCR Primer, Index 1 (100% over 23bp)
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC208660.10997040401606475RNA PCR Primer, Index 1 (100% over 24bp)
TGTGGCGTCGCTGAACCATAGCATGGAATTCTCGGGTGCCAAGGAACTCC201710.10630753471714954RNA PCR Primer, Index 1 (100% over 27bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAA198880.10481603541989341No Hit
CGCGCGCTACACTGACGGAGCCAGCGTGGAATTCTCGGGTGCCAAGGAAC197050.10385156767643805RNA PCR Primer, Index 1 (100% over 24bp)
CACGTTCTAGCATTCAAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT195550.10306102034573691RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC193770.10212290417997158RNA PCR Primer, Index 1 (100% over 26bp)
TTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTGCCAA192610.1015115475775627No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)