FastQCFastQC Report
Thu 29 Jun 2023
SRR1585897.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585897.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17670058
Total Bases706.8 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC1921431.087393148341675No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTC1320240.7471622334233424No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG1155600.6539876665939637No Hit
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGC895530.5068064858643927No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGG810910.45891756552242213No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGG554000.31352472074511584No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGG544680.30825026154413304No Hit
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGG523950.296518551325638No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTC515730.29186661413335485No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCG495680.280519735702056No Hit
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGG468400.26508118988630375No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC450380.25488314752560515No Hit
TCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGC414620.2346455229518771No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCG400960.22691493146202463No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG399450.2260603785228096No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGG378400.21414757099269283No Hit
CGCGCGCTACACTGACGGAGCCAGCGTGGAATTCTCGGGT369130.2089014082466509No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAAT361450.20455507276772944No Hit
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGT355640.20126702470359747No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGG354270.20049170183821696No Hit
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTG342140.19362698186955585No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTC341470.19324780937334785No Hit
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGG328820.18608880627330143No Hit
TCAGATTTAGGCTCTGATATGGAATTCTCGGGTGCCAAGG311870.17649630804833805Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTG311770.17643971513845624No Hit
TCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTG309620.17522296757599776No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTC304560.1723593663359792No Hit
TCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGG300650.17014658355960122No Hit
CCTGATTCAAGCTCAGGTATTGGAATTCTCGGGTGCCAAG283790.16060501895353144No Hit
TTTACCCAATCCTAATTCAGGGAGGTAGTGGAATTCTCGG264570.14972786167425145No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGG242630.13731137724618675No Hit
TTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGT218260.12351968510799455No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGG216280.12239914549233512No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCG208850.11819429228811812No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATT208690.11810374363230725No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGG202000.11431767796121553No Hit
TCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGG200570.11350839934990593No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCATGGAATTCT199780.11306131536183979No Hit
GGCGCCAGTGAAATACCACTACCTTGGAATTCTCGGGTGC197710.11188984212728674No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCT193100.10928090898173623No Hit
TGGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGG187540.10613434319230872No Hit
AGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGT184820.10459501604352403No Hit
TTTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTG183800.10401776836272976No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTC181330.10261992348864957No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCT180740.10228602532034699No Hit
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGT180730.1022803660293588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)