FastQCFastQC Report
Wed 28 Jun 2023
SRR1585900.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585900.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14907009
Total Bases745.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC12446508.349428111299858No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC5020573.36792578578305No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCA2840471.9054593714943087No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGCCA2466211.6543962642002832No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCCA1936501.2990533513463365No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCC1838521.2333258804633445No Hit
CACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC1816341.2184469735008545RNA PCR Primer, Index 1 (100% over 27bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGC1327090.8902456555838936No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGC1289990.865358033928872No Hit
CACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA1173600.7872806677717843RNA PCR Primer, Index 1 (100% over 28bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA1043600.7000733681719786No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG959130.6434087481935511Illumina Small RNA Adapter 2 (100% over 21bp)
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC952800.6391624235284221No Hit
TTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAACT693640.4653113176493018RNA PCR Primer, Index 1 (100% over 25bp)
TTGCTCGAATATATTAGCATGGAATAATAGATGGAATTCTCGGGTGCCAA679030.4555105588250467No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAA665790.44662883077349724No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGGA625110.41933965425257336RNA PCR Primer, Index 1 (100% over 22bp)
TTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTGCCA577480.38738824132996763No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG532700.3573486807447423Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAA532460.3571876826531734No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGTGC497520.3337490438222718No Hit
ATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACTCC489320.32824827569366866RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACTC454150.3046553470250135RNA PCR Primer, Index 1 (100% over 26bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCGGGTGCCAAG453780.30440714163384486No Hit
TCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC437020.2931641082392853RNA PCR Primer, Index 1 (100% over 26bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTGCC429820.28833416549221913No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG400120.2684106516605712No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTGCCAA385530.2586233093439469No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCA383370.257174326519827No Hit
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTC368810.24740710896464876RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAAGGA353700.23727093744962519RNA PCR Primer, Index 1 (100% over 22bp)
CACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG332760.2232238539602411RNA PCR Primer, Index 1 (100% over 29bp)
TCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCA328410.2203057635505553RNA PCR Primer, Index 1 (100% over 28bp)
GTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC301530.20227397729484167No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG299510.20091891002413698No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGA291350.19544497491079532RNA PCR Primer, Index 1 (100% over 22bp)
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGGA286360.19209755625692584RNA PCR Primer, Index 1 (100% over 22bp)
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT284900.19111815119988187RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAACCCATCTCGTATGC283310.1900515388432381RNA PCR Primer, Index 9 (97% over 37bp)
TTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCC271700.18226325616359393RNA PCR Primer, Index 1 (100% over 27bp)
TCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCC271140.18188759394993323RNA PCR Primer, Index 1 (100% over 27bp)
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCGGGTGCCAAGG267460.17941895654587717Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGCC264140.17719181627917444No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATATGGAATTCTCGGGTGCCAAG252790.16957794819872987No Hit
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA248700.1668342723882437RNA PCR Primer, Index 1 (100% over 23bp)
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGAA226960.15225052859363003RNA PCR Primer, Index 1 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATAGTGGAATTCTCGGGTGCCAAG225980.15159311971972378No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA220450.14788345535982436No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATGGAATTCTCGGGTGCCAA218930.14686380077988817No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGGAATTCTCGGGTGCCA217030.14558923255496792No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG214020.14357004815654167No Hit
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC208460.13984025903519612RNA PCR Primer, Index 1 (100% over 24bp)
TTGCTCGAATATATTAGCATGGAATAATGGAATTCTCGGGTGCCAAGGAA208410.13980671776611928RNA PCR Primer, Index 1 (100% over 23bp)
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAA206970.13884072921670604No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA199330.13371562330176362RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTCGAATATATTAGCATGGATGGAATTCTCGGGTGCCAAGGAACTCC190110.1275306132839928RNA PCR Primer, Index 1 (100% over 27bp)
GCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGAA184310.12363982607107837RNA PCR Primer, Index 1 (100% over 23bp)
CAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAGG183510.1231031657658488Illumina Small RNA Adapter 2 (100% over 21bp)
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC182140.12218413499314316No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAGGA178560.11978258012724083RNA PCR Primer, Index 1 (100% over 22bp)
GAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAA173270.11623391385891026RNA PCR Primer, Index 1 (100% over 23bp)
GTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCAAGG168750.11320178313436316Illumina Small RNA Adapter 2 (100% over 21bp)
CTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGAA167740.11252424949901084RNA PCR Primer, Index 1 (100% over 23bp)
TATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACTC163920.10996169654153962RNA PCR Primer, Index 1 (100% over 26bp)
CGCGCGCTACACTGACGGAGCCAGCGTGGAATTCTCGGGTGCCAAGGAAC162290.10886825116963436RNA PCR Primer, Index 1 (100% over 24bp)
CAAGACTGTAAATCTTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC161380.10825780007243573RNA PCR Primer, Index 1 (100% over 31bp)
ATGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC157760.1058294121912719No Hit
GGCGCCAGTGAAATACCACTACCTTGGAATTCTCGGGTGCCAAGGAACTC156790.10517871157118105RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTCGAATATATTAGCATGGAATATGGAATTCTCGGGTGCCAAGGAAC155750.1044810531743826RNA PCR Primer, Index 1 (100% over 24bp)
TATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACTCCA153940.10326685923380068RNA PCR Primer, Index 1 (100% over 28bp)
CCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCATGGAATTCTCGGGTGCC153140.1027301989285711No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAGG152820.10251553480647929Illumina Small RNA Adapter 2 (100% over 21bp)
GGAATGAGTACAATGTAAATACCTTAACGAGGTGGAATTCTCGGGTGCCA150900.10122755007392831No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)