FastQCFastQC Report
Sun 2 Jul 2023
SRR1585901.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585901.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18255341
Total Bases730.2 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC2132261.1680198140368894No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTC1028760.563539185600532No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG896910.4913137475766681No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGG672690.36848941906919186No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG487030.26678767600123166No Hit
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGG467450.25606204781384256No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGG425380.23301673740304274No Hit
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGG423370.23191568976991447No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC421830.23107210103607487No Hit
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTG376550.20626840112162245No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTC367070.20107540034448002No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGG360200.19731211813572808No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCG355730.19486351966802481No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGG316580.17341774114216765No Hit
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGT309700.16964898108449467No Hit
CGCGCGCTACACTGACGGAGCCAGCGTGGAATTCTCGGGT307890.1686574904297871No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGG283630.155368228947353No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTC274660.15045459846518341No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCG263600.14439609755851726No Hit
TCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGG263080.14411124941462336No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGG249130.13646965016977772No Hit
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGG239720.13131499433508254No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTC221760.12147677767290133No Hit
TTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGT216390.11853517280230481No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCG215560.11808051134185879No Hit
GCCAAGGACGTTTTCATTAATCAAGAACTGGAATTCTCGG198400.10868052259336049No Hit
TCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGG195610.10715220274439136No Hit
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGC193630.10606758865802615No Hit
ACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTG188020.10299451541332479No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTC182900.1001898567657542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)