FastQCFastQC Report
Sun 2 Jul 2023
SRR1585904.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585904.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20072401
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC214754310.698984142455105No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC7998313.9847300778815646No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCA4992982.487485179276759No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCC3923671.9547586758554694No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGCCA3833381.9097765135321878No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCCA3617361.8021561047928447No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA2179441.0857893881255163No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGC1679820.836880450923634No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAA1627620.8108745934280608No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGC1593960.7941052991119498No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG1445520.7201530100958027Illumina Small RNA Adapter 2 (100% over 21bp)
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC1232480.6140172269376245No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTGCCA1220120.6078595181513163No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGGA1023270.5097895363887958RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGTGC857340.4271237905221204No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG821760.4093979589188159Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAA815650.4063539782809242No Hit
TTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAACT803380.40024110717995326RNA PCR Primer, Index 1 (100% over 25bp)
TTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTGCCAA745060.3711862870814508No Hit
TTGCTCGAATATATTAGCATGGAATAATAGATGGAATTCTCGGGTGCCAA680050.33879853237288354No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG652330.32498852528902744No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCGGGTGCCAAG630190.31395845469607747No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCA608440.30312268073958865No Hit
GTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC559760.27887047493720357No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTGCC539590.2688218514566344No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG527190.2626442148101764No Hit
CACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC507140.25265537491005685RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAAGGA462700.2305155222835574RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACTC451220.2247962264205463RNA PCR Primer, Index 1 (100% over 26bp)
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGA429340.21389568691857044RNA PCR Primer, Index 1 (100% over 22bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA423910.2111904799032263RNA PCR Primer, Index 1 (100% over 22bp)
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGGA421760.21011935742017113RNA PCR Primer, Index 1 (100% over 22bp)
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCGGGTGCCAAGG403130.20083795655537173Illumina Small RNA Adapter 2 (100% over 21bp)
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA391160.1948745444055248No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATATGGAATTCTCGGGTGCCAAG378790.18871185365417917No Hit
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA370290.184477183372333RNA PCR Primer, Index 1 (100% over 23bp)
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC368410.18354057394528936No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG365540.18211074997953658No Hit
ATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACTCC365300.18199118281863738RNA PCR Primer, Index 1 (100% over 27bp)
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAA360750.1797243887265903No Hit
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT358630.17866821213864748RNA PCR Primer, Index 1 (100% over 25bp)
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC332220.16551084247469947RNA PCR Primer, Index 1 (100% over 24bp)
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGAA325100.16196368336802358RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTCAGAATCTCGTATGC319200.1590243239959186RNA PCR Primer, Index 40 (98% over 50bp)
GCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGAA316050.15745500500911674RNA PCR Primer, Index 1 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATGGAATTCTCGGGTGCCAAGGAA312660.15576611886141573RNA PCR Primer, Index 1 (100% over 23bp)
CAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAGG297330.14812876645898018Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAGGA296480.14770529943079555RNA PCR Primer, Index 1 (100% over 22bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGCC289920.14443713036621778No Hit
GAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAA282360.1406707647978934RNA PCR Primer, Index 1 (100% over 23bp)
CTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGAA280870.13992845200731094RNA PCR Primer, Index 1 (100% over 23bp)
ATGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC280450.13971920947573735No Hit
TTGCTCGAATATATTAGCATGGAATAATAGTGGAATTCTCGGGTGCCAAG273470.136241797879586No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGGAATTCTCGGGTGCCA272940.13597775373260032No Hit
GATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCCAAG269680.13435363313038634No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCAAGG258660.12886350765909868Illumina Small RNA Adapter 2 (100% over 21bp)
TTGCTCGAATATATTAGCATGGAATATGGAATTCTCGGGTGCCAAGGAAC248380.12374204760058352RNA PCR Primer, Index 1 (100% over 24bp)
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATGGAATTCTCGGGTGCCAA245730.12242182686565499No Hit
CTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC243520.12132081259237497No Hit
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAAC238830.11898427098980335RNA PCR Primer, Index 1 (100% over 24bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGG232320.11574101175041293Illumina Small RNA Adapter 2 (100% over 21bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTGGAATTCTCGGGTGCCAA230620.11489407769404367No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGA224750.11196966421705107RNA PCR Primer, Index 1 (100% over 22bp)
GCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAA223730.11146150378322953No Hit
CGCGCGCTACACTGACGGAGCCAGCGTGGAATTCTCGGGTGCCAAGGAAC223000.1110978203354945RNA PCR Primer, Index 1 (100% over 24bp)
GCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGAAC216820.10801896594234044RNA PCR Primer, Index 1 (100% over 24bp)
CACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA204160.10171179820490832RNA PCR Primer, Index 1 (100% over 28bp)
CGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGA201730.10050118070080406RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)