FastQCFastQC Report
Sat 1 Jul 2023
SRR1585905.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585905.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18159320
Total Bases726.3 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC1805670.9943489073379399No Hit
TCAGATTTAGGCTCTGATATGGAATTCTCGGGTGCCAAGG1564150.8613483324265446Illumina Small RNA Adapter 2 (100% over 21bp)
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGC1279270.7044702114396354No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTC1064260.5860682007916596No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG895810.49330591674137575No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGG658980.36288803765779776No Hit
TCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGC499330.2749717500435038No Hit
CGCGCGCTACACTGACGGAGCCAGCGTGGAATTCTCGGGT468930.2582310350828115No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG450400.24802690849657366No Hit
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTG440380.24250908073650332No Hit
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGG420060.23131923442067212No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGG397660.21898397076542514No Hit
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGG385700.21239782106378435No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTC381630.21015654771213899No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC371970.2048369652608137No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCG364340.2006352660782452No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGG318830.17557375496439293No Hit
CAAGACTGTAAATCTTGAGTGGAATTCTCGGGTGCCAAGG315290.17362434276173336Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAAT311230.17138857622421985No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGG304280.1675613404026142No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGG299620.16499516501719227No Hit
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGT292700.16118444963798204No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGG292640.1611514087531912No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTC280490.15446062958304607No Hit
TCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTG266570.14679514431157115No Hit
ACCTGCGGAAGGATCATTATGGAATTCTCGGGTGCCAAGG259920.14313311291391967Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCG254840.14033565133496187No Hit
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGG251110.138281609663798No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCT237450.13075930155975005No Hit
TCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGG220390.12136467665088782No Hit
ACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTG207660.11435450226109788No Hit
GGCGCCAGTGAAATACCACTACCTTGGAATTCTCGGGTGC203080.11183238138873042No Hit
TCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGG199430.10982239423062098No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTC198530.10932678095875836No Hit
TTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCC194310.10700290539513593No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTC192000.1057308313306886No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCT188930.10404023939222394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)