FastQCFastQC Report
Wed 28 Jun 2023
SRR1585908.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585908.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16875090
Total Bases843.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA8053214.772247140607843RNA PCR Primer, Index 1 (100% over 28bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC7198144.265541694888738No Hit
CACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG7124674.2220041493112035RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC5122723.0356697356873354RNA PCR Primer, Index 1 (100% over 27bp)
TGTGGCGTCGCTGAACCATAGTGGAATTCTCGGGTGCCAAGGAACTCCAG4001952.3715132778551107RNA PCR Primer, Index 1 (100% over 29bp)
TTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCCA2498031.4803061791077856No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCC2099621.2442126234586008No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC1774591.0516032803380604No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCA1604150.9506023375282739No Hit
GGCGCCAGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA1534950.909595148825873RNA PCR Primer, Index 1 (100% over 28bp)
GTGGCGTCGCTGAACCATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1512930.8965463295306869RNA PCR Primer, Index 1 (100% over 30bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGCCA1488550.882098999175708No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA1320590.7825676781575683No Hit
TCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCA1293570.7665559117018043RNA PCR Primer, Index 1 (100% over 28bp)
TCGGTGCCCGAGTTGTAATTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1270610.7529500583404296RNA PCR Primer, Index 1 (100% over 29bp)
TCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCC1120340.6639016443764152RNA PCR Primer, Index 1 (100% over 27bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAA1090630.6462958123482601No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTGCCA988590.585827986695182No Hit
TCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCA969900.5747524902089411RNA PCR Primer, Index 1 (100% over 28bp)
ACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCA962120.5701421444270816RNA PCR Primer, Index 1 (100% over 28bp)
ACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG923020.5469718976313608RNA PCR Primer, Index 1 (100% over 29bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGC836560.49573661533064417No Hit
TCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC764650.4531235092672098RNA PCR Primer, Index 1 (100% over 26bp)
CGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG699300.414397789878454RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCTGTATCTCGTATGC654640.38793274584017035RNA PCR Primer, Index 11 (97% over 37bp)
TTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTGCCAA604550.35824994118549885No Hit
ACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT593630.3517788645867963RNA PCR Primer, Index 1 (100% over 30bp)
TGTGGCGTCGCTGAACCATAGCTGGAATTCTCGGGTGCCAAGGAACTCCA567250.3361463553675862RNA PCR Primer, Index 1 (100% over 28bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG540620.32036569879034715Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATATGGAATTCTCGGGTGCCAAG539250.3195538512683488No Hit
AGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG520370.3083657627900058RNA PCR Primer, Index 1 (100% over 29bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGC497120.29458805849331765No Hit
TTCGGTGCCCGAGTTGTAATTTTGGAATTCTCGGGTGCCAAGGAACTCCA462520.2740844641421171RNA PCR Primer, Index 1 (100% over 28bp)
GTGGCGTCGCTGAACCATAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG458500.27170225462501235RNA PCR Primer, Index 1 (100% over 29bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG447880.2654089548559445Illumina Small RNA Adapter 2 (100% over 21bp)
GGCGCCAGTGAAATACCACTACCTGGAATTCTCGGGTGCCAAGGAACTCC430810.2552934532497308RNA PCR Primer, Index 1 (100% over 27bp)
TTGTGGCGTCGCTGAACCATAGTGGAATTCTCGGGTGCCAAGGAACTCCA423670.2510623647044253RNA PCR Primer, Index 1 (100% over 28bp)
GTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC421030.24949792860364003RNA PCR Primer, Index 1 (100% over 31bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTGCC420700.2493023740910419No Hit
TTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCC418820.24818830595866453RNA PCR Primer, Index 1 (100% over 27bp)
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGGA399560.23677503349611764RNA PCR Primer, Index 1 (100% over 22bp)
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC375450.22248770228781No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG367940.21803735565262172No Hit
GGGAATACTGCCAGCTGGGACTGAGGATGGAATTCTCGGGTGCCAAGGAA366610.21724921170790792RNA PCR Primer, Index 1 (100% over 23bp)
ATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG364890.21622995788466906RNA PCR Primer, Index 1 (100% over 29bp)
ATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACTCC361140.21400774751423549RNA PCR Primer, Index 1 (100% over 27bp)
TTTCGGTGCCCGAGTTGTAATTTTGGAATTCTCGGGTGCCAAGGAACTCC336660.19950115821604505RNA PCR Primer, Index 1 (100% over 27bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA320290.18980046921231236RNA PCR Primer, Index 1 (100% over 22bp)
CGGTGCCCGAGTTGTAATTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT307140.18200791817999193RNA PCR Primer, Index 1 (100% over 30bp)
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTC306730.1817649565128245RNA PCR Primer, Index 1 (100% over 26bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG306310.18151606895133598No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAA282780.1675724396136554No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCA270280.16016507171221014No Hit
TACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA267290.1583932293101844RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAA259430.15373547637375565No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA258370.15310733157571307No Hit
GTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA255860.15161993210110286RNA PCR Primer, Index 1 (100% over 32bp)
TTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAACT251470.1490184644941153RNA PCR Primer, Index 1 (100% over 25bp)
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGGA247870.14688514253849905RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGTGC246500.14607329501650065No Hit
AGCGGAGAATTTGGACAAACTTGGAATTCTCGGGTGCCAAGGAACTCCAG243750.14444367407818268RNA PCR Primer, Index 1 (100% over 29bp)
TTGATGATTCATAATAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA237410.14068665707856967RNA PCR Primer, Index 1 (100% over 32bp)
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC237060.1404792507773292RNA PCR Primer, Index 1 (100% over 24bp)
TACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG233460.13834592882171295RNA PCR Primer, Index 1 (100% over 29bp)
CAAGACTGTAAATCTTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC225580.13367632409664187RNA PCR Primer, Index 1 (100% over 31bp)
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA223080.1321948505163528RNA PCR Primer, Index 1 (100% over 23bp)
AATACTGCCAGCTGGGACTGAGGACTGCGACGTGGAATTCTCGGGTGCCA218620.12955190164911712No Hit
TTTGATGATTCATAATAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC213060.12625710440655427RNA PCR Primer, Index 1 (100% over 31bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG197500.11703641284283521No Hit
CCTGCGGAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA197250.11688826548480631RNA PCR Primer, Index 1 (100% over 32bp)
GTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC191480.11346902446149917No Hit
TTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACTC189700.11241421527233336RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTCGAATATATTAGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTC185810.11010904238140361RNA PCR Primer, Index 1 (100% over 31bp)
GTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT179880.10659498704895795RNA PCR Primer, Index 1 (100% over 30bp)
GGCGCCAGTGAAATACCACTACCTTGGAATTCTCGGGTGCCAAGGAACTC173070.10255945301625058RNA PCR Primer, Index 1 (100% over 26bp)
AGCGGAGAATTTGGACAAACTTTGGAATTCTCGGGTGCCAAGGAACTCCA172750.1023698243979736RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT169040.10017131760482463RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)