FastQCFastQC Report
Wed 28 Jun 2023
SRR1585909.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585909.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17546901
Total Bases701.8 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC2566431.4626115460502114No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTC680630.38789185623147926No Hit
TCAGATTTAGGCTCTGATATGGAATTCTCGGGTGCCAAGG618690.35259217567820095Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG598820.34126823876193296No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGG578880.32990440876141036No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGG572150.32606897366093307No Hit
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGT503530.2869623530673593No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC453540.25847299189754364No Hit
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGG438890.25012393926426096No Hit
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGG434900.24785003346174916No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG397230.22638185512074183No Hit
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTG369490.2105727957318503No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTC320320.182550753549017No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCG317060.18069287562516026No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCG271420.1546825846911657No Hit
GGGAATACTGCCAGCTGGGACTGAGGATGGAATTCTCGGG249120.14197378785005968No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGG248490.14161475009176833No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAAT245960.14017290004656663No Hit
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGC240110.13683897800528994No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGG235930.1344567909740871No Hit
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGG211460.1205113085210887No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTC209850.11959376758323306No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTC204200.11637382578268379No Hit
TTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGT199880.11391185258297178No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCG192540.10972877774827589No Hit
GCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGG189290.1078765988364555No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCG178270.10159628757237531No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)