FastQCFastQC Report
Wed 28 Jun 2023
SRR1585912.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585912.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16108623
Total Bases805.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC5294443.2867117195554205No Hit
CACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC3040151.8872811164554537RNA PCR Primer, Index 1 (100% over 27bp)
TTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCCA2364471.467828752339663No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGCCA1876131.164674348639235No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCA1420180.8816271881215421No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCC1258700.7813827414050227No Hit
CACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA1252210.7773538433421653RNA PCR Primer, Index 1 (100% over 28bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAA1120030.6952984125334611No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG1057700.6566048507063578Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTGCCA1016990.6313326719484341No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGGA929580.5770698091326614RNA PCR Primer, Index 1 (100% over 22bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA842580.523061468382493No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTGCCAA810080.5028859387919128No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG796640.4945425813243007Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGA788010.48918520223609435RNA PCR Primer, Index 1 (100% over 22bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC758700.4709899784730204No Hit
TCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC616960.3829998380370563RNA PCR Primer, Index 1 (100% over 26bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAGG613210.38067189231506626Illumina Small RNA Adapter 2 (100% over 21bp)
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG567130.35206609528325294No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTGC561460.34854624135160406No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA541730.3362981429263072RNA PCR Primer, Index 1 (100% over 22bp)
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC526710.3269739443278299RNA PCR Primer, Index 1 (100% over 24bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTGCC487750.30278814024016826No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAA474590.29461860271979795No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATATGGAATTCTCGGGTGCCAAG466930.2898633855916797No Hit
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTC466450.289565408539265RNA PCR Primer, Index 1 (100% over 26bp)
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT434410.2696754402905822RNA PCR Primer, Index 1 (100% over 25bp)
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAAC421110.26141899279659103RNA PCR Primer, Index 1 (100% over 24bp)
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGA415470.257917762430718RNA PCR Primer, Index 1 (100% over 22bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGC410110.25459035201208696No Hit
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGGA405880.2519644292376822RNA PCR Primer, Index 1 (100% over 22bp)
GGGAATACTGCCAGCTGGGACTGAGGATGGAATTCTCGGGTGCCAAGGAA387700.24067854837747457RNA PCR Primer, Index 1 (100% over 23bp)
GCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGAA387330.24044885773290495RNA PCR Primer, Index 1 (100% over 23bp)
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA385930.23957975799669531RNA PCR Primer, Index 1 (100% over 23bp)
GTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCAAGG376520.233738166198315Illumina Small RNA Adapter 2 (100% over 21bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG375060.23283181933055358No Hit
GGCGCCAGTGAAATACCACTACCTGGAATTCTCGGGTGCCAAGGAACTCC356230.2211424278785344RNA PCR Primer, Index 1 (100% over 27bp)
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAA339100.21050837182048396No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC333580.20708163571771468No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG327520.20331967543097879No Hit
TTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAACT318100.19747187577733985RNA PCR Primer, Index 1 (100% over 25bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCGGGTGCCAAG309810.19232556376792725No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCAAG309440.1920958731233576No Hit
GGCGCCAGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA303130.18817871645515571RNA PCR Primer, Index 1 (100% over 28bp)
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGAA293980.18249852889350007RNA PCR Primer, Index 1 (100% over 23bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTGGAATTCTCGGGTGCCAAGGA292040.1812942049733239RNA PCR Primer, Index 1 (100% over 22bp)
CTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGAA284310.17649553285839517RNA PCR Primer, Index 1 (100% over 23bp)
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA270550.16795352402250643No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGG268260.16653192516827786Illumina Small RNA Adapter 2 (100% over 21bp)
CACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG266740.1655883311689646RNA PCR Primer, Index 1 (100% over 29bp)
TCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCC264180.16399912022275273RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACTC257190.15965982939696335RNA PCR Primer, Index 1 (100% over 26bp)
TGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCA255700.15873485896342598No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGA255620.15868519612135687RNA PCR Primer, Index 1 (100% over 22bp)
GGCGCCAGTGAAATACCACTACCTTGGAATTCTCGGGTGCCAAGGAACTC254250.15783471995092319RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTCGAATATATTAGCATGGAATAATAGATGGAATTCTCGGGTGCCAA252950.1570276987673No Hit
CCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCATGGAATTCTCGGGTGCC250430.1554633192421227No Hit
GCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGAAC249640.15497289867669012RNA PCR Primer, Index 1 (100% over 24bp)
ATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACTCC244730.15192484174469786RNA PCR Primer, Index 1 (100% over 27bp)
TTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCC244380.15170756681064546RNA PCR Primer, Index 1 (100% over 27bp)
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCGGGTGCCAAGG236590.14687164756416485Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACGGTGATCTCGTATGC226050.14032856812155825RNA PCR Primer, Index 5 (98% over 50bp)
TCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCA219990.13656660783482238RNA PCR Primer, Index 1 (100% over 28bp)
TTGCTCGAATATATTAGCATGGATGGAATTCTCGGGTGCCAAGGAACTCC201680.12520002485625245RNA PCR Primer, Index 1 (100% over 27bp)
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA189520.11765127286174615RNA PCR Primer, Index 1 (100% over 23bp)
CACGTTCTAGCATTCAAGGTCCCGTGGAATTCTCGGGTGCCAAGGAACTC189200.11745262149346967RNA PCR Primer, Index 1 (100% over 26bp)
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCTCG187370.11631658398113855No Hit
CGCGCGCTACACTGACGGAGCCAGCGTGGAATTCTCGGGTGCCAAGGAAC181560.11270982007586867RNA PCR Primer, Index 1 (100% over 24bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAAG173590.10776215943473257No Hit
TGTGGCGTCGCTGAACCATAGCAGTGGAATTCTCGGGTGCCAAGGAACTC164860.1023427017939398RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)