FastQCFastQC Report
Wed 28 Jun 2023
SRR1585913.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585913.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19038456
Total Bases761.5 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC2454581.2892747184960798No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTC1522840.799875788246694No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG1307960.6870094927866No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGG1226610.6442801874269637No Hit
TCAGATTTAGGCTCTGATATGGAATTCTCGGGTGCCAAGG1207370.634174325901218Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG869540.4567282136744702No Hit
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGT739620.38848738574178493No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCG520190.2732311905965484No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGG495320.2601681564933627No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGG470510.2471366375508602No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTC456170.23960451414757586No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCG435140.22855845032811484No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGG413190.21702915404484482No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC411450.21611521438503206No Hit
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTG407000.21377783996769487No Hit
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGG403770.2120812738175827No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTC377700.19838793650073303No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCG369680.19417541002274555No Hit
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGG364010.1911972273381833No Hit
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGG358830.18847641846586718No Hit
GCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGG358020.18805096379664402No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGG334120.17549742479117003No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGG272140.14294226380542624No Hit
CAAGACTGTAAATCTTGAGTGGAATTCTCGGGTGCCAAGG270850.14226468785073748Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTC270360.14200731403849134No Hit
GCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGT268770.14117216228038662No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTC267650.1405838792809669No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGG261680.13744812079298868No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAAT259680.13639761543688206No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCG252800.13278387701187533No Hit
TCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGG250310.1314759978435226No Hit
GGGAATACTGCCAGCTGGGACTGAGGATGGAATTCTCGGG230390.12101296449670079No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCG226700.11907478211468409No Hit
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGC208170.10934184999035634No Hit
ACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTG207810.10915275902625718No Hit
TTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGT203950.10712528368897142No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTGGAATTCTCG198050.10402629288845693No Hit
TGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGG197950.1039737676206516No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCT190870.10025497866003419No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)