FastQCFastQC Report
Wed 28 Jun 2023
SRR1585921.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585921.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18958390
Total Bases663.5 Mbp
Sequences flagged as poor quality0
Sequence length35
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAA2415041.2738634451554165No Hit
CACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAA1806370.9528077014978592No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAA1686690.8896799780994061No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAA1015860.5358366401366361No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAA962530.5077066143274824No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAA771670.4070335086470951No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATA660220.3482468711741873No Hit
CCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAA629790.3321959301396374No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCAAAAAAAA519150.27383654413692304No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAA502660.26513854815730664No Hit
GGCGCCAGTGAAATACCACTACCTTTAAAAAAAAA459080.24215136411899957No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATAAA458340.24176103561536605No Hit
TTTCCACGTTCTAGCATTCAAGGTCCAAAAAAAAA454310.23963532768341614No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAA448950.23680808338682768No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAA421600.22238175288091447No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAA417500.22021912198240462No Hit
TTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAA413110.21790352450814654No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTAAAAAA356510.18804866868969358No Hit
GGCGCCAGTGAAATACCACTACCTTTATAAAAAAA337180.17785265520964597No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAA337120.17782100695259462No Hit
GCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAA331350.17477749956615513No Hit
GCTCGAATATATTAGCATGGAATAATAGAATAAAA304660.16069929988780693No Hit
CCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAA288710.1522861382216528No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTAAAAAA270080.14245935440720442No Hit
TCCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAA238410.12575434939359303No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAAA237890.12548006449914786No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTAAAAA234600.12374468507083144No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACAA232990.12289545683995319No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAA225110.11873898574720743No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAA218560.11528405101910025No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAA218500.11525240276204889No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTAAAAAAA210260.11090604212699495No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAAAAA209530.11052098833287004No Hit
AGCCATGCATGTCTAAGTATAAGCAAAAAAAAAAA205290.10828451150124035No Hit
TTTTCCACGTTCTAGCATTCAAGGTCAAAAAAAAA197030.1039276014471693No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAA194350.10251397929887507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)