FastQCFastQC Report
Wed 28 Jun 2023
SRR1585924.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585924.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13073197
Total Bases653.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC2491561.9058536332008154RNA PCR Primer, Index 1 (100% over 26bp)
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG1013140.774974935358199No Hit
TCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAACT992980.7595540708213913RNA PCR Primer, Index 1 (100% over 25bp)
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTC919560.7033933627711721RNA PCR Primer, Index 1 (100% over 26bp)
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA610440.46694010654012175RNA PCR Primer, Index 1 (100% over 23bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAG584810.44733510861956716No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACT527140.40322195098872904RNA PCR Primer, Index 1 (100% over 25bp)
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGA472020.3610593491400764RNA PCR Primer, Index 1 (100% over 22bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG435750.3333155616028734Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAG430870.3295827332824557No Hit
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA406670.31107157644759736RNA PCR Primer, Index 1 (100% over 23bp)
TTCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACT406150.3106738160527987RNA PCR Primer, Index 1 (100% over 25bp)
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGGA390950.2990469737433009RNA PCR Primer, Index 1 (100% over 22bp)
CCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC377850.28902647148972055RNA PCR Primer, Index 1 (100% over 26bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAGG376800.2882233014617618Illumina Small RNA Adapter 2 (100% over 21bp)
TCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCC360500.2757550429324977RNA PCR Primer, Index 1 (100% over 27bp)
CCTGATTCAAGCTCAGGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC305960.23403609690881275RNA PCR Primer, Index 1 (100% over 31bp)
CCTGATTCAAGCTCAGGTATTGGAATTCTCGGGTGCCAAGGAACTCCAGT290590.22227921754716923RNA PCR Primer, Index 1 (100% over 30bp)
ACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTGCCAAGGAACT289060.22110888407785792RNA PCR Primer, Index 1 (100% over 25bp)
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT261620.20011937401386973RNA PCR Primer, Index 1 (100% over 25bp)
GCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC242640.1856011196037205RNA PCR Primer, Index 1 (100% over 26bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG234100.1790686700429895No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGC230150.17604722089019234No Hit
GGCGCCAGTGAAATACCACTACCTTGGAATTCTCGGGTGCCAAGGAACTC216440.16556011509655977RNA PCR Primer, Index 1 (100% over 26bp)
TAGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGA212610.1626304568041008RNA PCR Primer, Index 1 (100% over 22bp)
TCACGTTCTAGCATTCAAGGTCCCGTGGAATTCTCGGGTGCCAAGGAACT208470.15946367212243492RNA PCR Primer, Index 1 (100% over 25bp)
TCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA207400.1586452036177532RNA PCR Primer, Index 1 (100% over 23bp)
TCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTC201720.15430043622841452RNA PCR Primer, Index 1 (100% over 26bp)
TTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAAC198970.1521968956789988RNA PCR Primer, Index 1 (100% over 24bp)
TGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCAAG191330.14635287757080384No Hit
TGGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGA188730.1443640755968108RNA PCR Primer, Index 1 (100% over 22bp)
TGGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGG183020.1399963604923876Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGG175910.13455775201735276Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAA174760.1336780896057789RNA PCR Primer, Index 1 (100% over 23bp)
TCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGA173800.1329437627230738RNA PCR Primer, Index 1 (100% over 22bp)
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAA173150.13244656222957552No Hit
CACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC172260.13176578001540099RNA PCR Primer, Index 1 (100% over 27bp)
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGA169950.12999880595389177RNA PCR Primer, Index 1 (100% over 22bp)
TCAGATTTAGGCTCTGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTC167930.1284536598048664RNA PCR Primer, Index 1 (100% over 31bp)
TTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAGGA167190.12788761616611455RNA PCR Primer, Index 1 (100% over 22bp)
TTTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACT166580.1274210126260623RNA PCR Primer, Index 1 (100% over 25bp)
AGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAAC165240.12639601468561976RNA PCR Primer, Index 1 (100% over 24bp)
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC164060.12549340455896135RNA PCR Primer, Index 1 (100% over 24bp)
TGCAAGAGTTCGAATCTCTTAGCAACCACCATGGAATTCTCGGGTGCCAA159090.12169173309329002No Hit
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGAA159070.121676434616567RNA PCR Primer, Index 1 (100% over 23bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCGGGTGCCAA158320.12110274173945364No Hit
TTCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAAC155960.11929752148613687RNA PCR Primer, Index 1 (100% over 24bp)
TTTACCCAATCCTAATTCAGGGAGGTAGTGGAATTCTCGGGTGCCAAGGA152910.11696450378587579RNA PCR Primer, Index 1 (100% over 22bp)
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGGA144760.11073037452124374RNA PCR Primer, Index 1 (100% over 22bp)
AGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAAC139360.1065997858060274RNA PCR Primer, Index 1 (100% over 24bp)
CCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACT138680.1060796375974446RNA PCR Primer, Index 1 (100% over 25bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA138120.10565128024919994RNA PCR Primer, Index 1 (100% over 22bp)
TGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG137300.10502404270355599Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGA135450.10360893360667632RNA PCR Primer, Index 1 (100% over 22bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG132880.10164307934776781No Hit
TTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAAC131770.10079401388963999RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)