FastQCFastQC Report
Wed 28 Jun 2023
SRR1585925.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585925.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12771510
Total Bases638.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG1525931.1947921584840007No Hit
TCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC1243830.9739098978899128RNA PCR Primer, Index 1 (100% over 26bp)
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTC1075790.8423357927136259RNA PCR Primer, Index 1 (100% over 26bp)
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA680210.5325995125087011RNA PCR Primer, Index 1 (100% over 23bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAG627940.4916724803879886No Hit
TCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAACT527440.4129817069398998RNA PCR Primer, Index 1 (100% over 25bp)
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGA488600.38257026772871805RNA PCR Primer, Index 1 (100% over 22bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG450970.3531062497699959Illumina Small RNA Adapter 2 (100% over 21bp)
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA379460.29711443674240556RNA PCR Primer, Index 1 (100% over 23bp)
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGGA365130.2858941503393099RNA PCR Primer, Index 1 (100% over 22bp)
CCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAG332490.26033726630602017No Hit
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT313600.24554653286886202RNA PCR Primer, Index 1 (100% over 25bp)
TCAGATTTAGGCTCTGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTC299330.23437322603200406RNA PCR Primer, Index 1 (100% over 31bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAGG290850.22773344733708073Illumina Small RNA Adapter 2 (100% over 21bp)
TCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA286930.22466411567621997RNA PCR Primer, Index 1 (100% over 23bp)
CCTGATTCAAGCTCAGGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC284060.22241692642451832RNA PCR Primer, Index 1 (100% over 31bp)
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGGA267380.20935660701044745RNA PCR Primer, Index 1 (100% over 22bp)
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC255690.2002034215218091RNA PCR Primer, Index 1 (100% over 24bp)
CGCGCGCTACACTGACGGAGCCAGCGTGGAATTCTCGGGTGCCAAGGAAC249040.19499651959713457RNA PCR Primer, Index 1 (100% over 24bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGC246090.19268669092378268No Hit
CCTGATTCAAGCTCAGGTATTGGAATTCTCGGGTGCCAAGGAACTCCAGT229960.18005701753355713RNA PCR Primer, Index 1 (100% over 30bp)
TCACGTTCTAGCATTCAAGGTCCCGTGGAATTCTCGGGTGCCAAGGAACT220550.17268905556195RNA PCR Primer, Index 1 (100% over 25bp)
TAGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGA219420.17180427373114066RNA PCR Primer, Index 1 (100% over 22bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGG217530.17032441739465418Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA212880.16668350101123516RNA PCR Primer, Index 1 (100% over 22bp)
TGGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGA202070.1582193491607492RNA PCR Primer, Index 1 (100% over 22bp)
ACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTGCCAAGGAACT200130.15670034318573137RNA PCR Primer, Index 1 (100% over 25bp)
GGCGCCAGTGAAATACCACTACCTTGGAATTCTCGGGTGCCAAGGAACTC199430.15615224824629195RNA PCR Primer, Index 1 (100% over 26bp)
TTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAAC197130.15435136487384812RNA PCR Primer, Index 1 (100% over 24bp)
TGGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGG196720.15403033783789075Illumina Small RNA Adapter 2 (100% over 21bp)
TCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACT193010.1511254346588618RNA PCR Primer, Index 1 (100% over 25bp)
TCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGA191420.14988047615356367RNA PCR Primer, Index 1 (100% over 22bp)
TGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG186150.14575410425235544Illumina Small RNA Adapter 2 (100% over 21bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG185290.1450807304696156No Hit
TTTACCCAATCCTAATTCAGGGAGGTAGTGGAATTCTCGGGTGCCAAGGA183400.14360087413312914RNA PCR Primer, Index 1 (100% over 22bp)
CCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC171390.13419713095788988RNA PCR Primer, Index 1 (100% over 26bp)
TGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCAAG164980.129178147298166No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGA164350.1286848618526705RNA PCR Primer, Index 1 (100% over 22bp)
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGAA163680.12816025669634992RNA PCR Primer, Index 1 (100% over 23bp)
AGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAAC158120.12380681689165964RNA PCR Primer, Index 1 (100% over 24bp)
TTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAGGA152840.11967261506274512RNA PCR Primer, Index 1 (100% over 22bp)
AGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAAC148150.11600037896850099RNA PCR Primer, Index 1 (100% over 24bp)
TTTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACT143030.11199145598288691RNA PCR Primer, Index 1 (100% over 25bp)
TCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCC137520.10767716581672802RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC134000.10492103126411834RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC132490.10373871218047044No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCATGGAATTCTCGGGTGCCAA132290.10358211362634488No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACGGTGATCTCGTATGC129190.10115483603739886RNA PCR Primer, Index 5 (98% over 50bp)
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAAC128400.10053627174860294RNA PCR Primer, Index 1 (100% over 24bp)
ATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCAAGGAACT127980.10020741478493927RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)