FastQCFastQC Report
Wed 28 Jun 2023
SRR1585926.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585926.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12828056
Total Bases641.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG1651451.2873735505987813No Hit
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTC608130.4740624768086451RNA PCR Primer, Index 1 (100% over 26bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAG587890.4582845600299843No Hit
TCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC540570.4213966636877793RNA PCR Primer, Index 1 (100% over 26bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG483160.3766431951965286Illumina Small RNA Adapter 2 (100% over 21bp)
TCAGATTTAGGCTCTGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTC403560.31459170430811967RNA PCR Primer, Index 1 (100% over 31bp)
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGGA390230.3042004182083396RNA PCR Primer, Index 1 (100% over 22bp)
TCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCC379360.2957268038118948RNA PCR Primer, Index 1 (100% over 27bp)
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGGA313160.2441211669172632RNA PCR Primer, Index 1 (100% over 22bp)
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGA296810.23137566596216916RNA PCR Primer, Index 1 (100% over 22bp)
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA292840.22828088683117692RNA PCR Primer, Index 1 (100% over 23bp)
CGCGCGCTACACTGACGGAGCCAGCGTGGAATTCTCGGGTGCCAAGGAAC278110.21679824285144997RNA PCR Primer, Index 1 (100% over 24bp)
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC277040.21596413361463343RNA PCR Primer, Index 1 (100% over 24bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA276830.21580042993264137RNA PCR Primer, Index 1 (100% over 22bp)
TCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCA273320.213064239819346RNA PCR Primer, Index 1 (100% over 28bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGC272210.21219894892881663No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA261710.20401376482921496RNA PCR Primer, Index 1 (100% over 23bp)
CCTGATTCAAGCTCAGGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC260430.20301595191040636RNA PCR Primer, Index 1 (100% over 31bp)
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT258120.201215211408494RNA PCR Primer, Index 1 (100% over 25bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG248040.1933574346728764No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG236540.1843927092304555Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGG205560.16024251842991644Illumina Small RNA Adapter 2 (100% over 21bp)
TCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA195890.15270435364485468RNA PCR Primer, Index 1 (100% over 23bp)
TCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGA191230.14907169098731718RNA PCR Primer, Index 1 (100% over 22bp)
TCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAACT185900.1449167356300908RNA PCR Primer, Index 1 (100% over 25bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC179170.13967042239291752No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGA176620.13768259196872853RNA PCR Primer, Index 1 (100% over 22bp)
TTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAAC173540.1352816046328454RNA PCR Primer, Index 1 (100% over 24bp)
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGA170220.13269352737468562RNA PCR Primer, Index 1 (100% over 22bp)
GGCGCCAGTGAAATACCACTACCTTGGAATTCTCGGGTGCCAAGGAACTC162660.12680019482297242RNA PCR Primer, Index 1 (100% over 26bp)
TGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCAAG155440.12117190632781771No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAG146480.1141872158961576No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCGGGTGCCAAGG146360.1140936709350193Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGAA144580.11270608734480113RNA PCR Primer, Index 1 (100% over 23bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG139080.10841860995929548No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAGG134970.10521469504030853Illumina Small RNA Adapter 2 (100% over 21bp)
TGGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGG129480.10093501306823106Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)