FastQCFastQC Report
Wed 28 Jun 2023
SRR1585927.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585927.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15939470
Total Bases796.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG1518160.9524532497002725No Hit
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTC1231250.7724535382920511RNA PCR Primer, Index 1 (100% over 26bp)
TCAGATTTAGGCTCTGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1066990.6694011783327801RNA PCR Primer, Index 1 (100% over 31bp)
TCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC764740.47977755847590914RNA PCR Primer, Index 1 (100% over 26bp)
CCTGATTCAAGCTCAGGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC504110.3162652208636799RNA PCR Primer, Index 1 (100% over 31bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAG477780.29974647839608215No Hit
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGGA477570.29961472997533795RNA PCR Primer, Index 1 (100% over 22bp)
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA457380.2869480603809286RNA PCR Primer, Index 1 (100% over 23bp)
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT426790.26775670709251936RNA PCR Primer, Index 1 (100% over 25bp)
TCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAACT424950.2666023399774271RNA PCR Primer, Index 1 (100% over 25bp)
CGCGCGCTACACTGACGGAGCCAGCGTGGAATTCTCGGGTGCCAAGGAAC375670.2356853772427816RNA PCR Primer, Index 1 (100% over 24bp)
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGGA353840.221989815219703RNA PCR Primer, Index 1 (100% over 22bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG345750.21691436415388968Illumina Small RNA Adapter 2 (100% over 21bp)
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGA309630.19425363578588248RNA PCR Primer, Index 1 (100% over 22bp)
GGCGCCAGTGAAATACCACTACCTTGGAATTCTCGGGTGCCAAGGAACTC305890.19190725914977097RNA PCR Primer, Index 1 (100% over 26bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA283840.17807367497162704RNA PCR Primer, Index 1 (100% over 22bp)
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC278290.17459175242338673RNA PCR Primer, Index 1 (100% over 24bp)
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA271380.1702566020074695RNA PCR Primer, Index 1 (100% over 23bp)
TTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAAC235040.1474578514843969RNA PCR Primer, Index 1 (100% over 24bp)
TGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG231960.14552554131348155Illumina Small RNA Adapter 2 (100% over 21bp)
TCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCC221710.13909496363429902RNA PCR Primer, Index 1 (100% over 27bp)
TCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGA215470.1351801534178991RNA PCR Primer, Index 1 (100% over 22bp)
TCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA210620.13213739227213955RNA PCR Primer, Index 1 (100% over 23bp)
TGGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGG206900.12980356310467034Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGG201930.12668551714705698Illumina Small RNA Adapter 2 (100% over 21bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG195590.12270796958744551No Hit
TGGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGA195490.12264523224423396RNA PCR Primer, Index 1 (100% over 22bp)
TTTTCCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAAC192340.12066900593307056RNA PCR Primer, Index 1 (100% over 24bp)
TAGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGA190720.11965266097304365RNA PCR Primer, Index 1 (100% over 22bp)
TTTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACT190040.1192260470392052RNA PCR Primer, Index 1 (100% over 25bp)
TTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAGGA186550.11703651376112253RNA PCR Primer, Index 1 (100% over 22bp)
TCACGTTCTAGCATTCAAGGTCCCGTGGAATTCTCGGGTGCCAAGGAACT169250.10618295338552662RNA PCR Primer, Index 1 (100% over 25bp)
TTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAA168600.10577516065465163RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)