FastQCFastQC Report
Wed 28 Jun 2023
SRR1585928.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585928.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17776048
Total Bases888.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG3025611.7020712365313144No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA720670.4054163220081314RNA PCR Primer, Index 1 (100% over 22bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAG650510.36594748168996843No Hit
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGGA571420.321455027574183RNA PCR Primer, Index 1 (100% over 22bp)
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGGA552660.3109015007160197RNA PCR Primer, Index 1 (100% over 22bp)
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA549110.3089044314011753RNA PCR Primer, Index 1 (100% over 23bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG522200.2937660834399187Illumina Small RNA Adapter 2 (100% over 21bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG484880.27277154067090725No Hit
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC439470.2472259300829971RNA PCR Primer, Index 1 (100% over 24bp)
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT379390.21342764150951887RNA PCR Primer, Index 1 (100% over 25bp)
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGA370050.20817338026990023RNA PCR Primer, Index 1 (100% over 22bp)
TGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG320620.18036629964095507Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGG230390.12960698575971444Illumina Small RNA Adapter 2 (100% over 21bp)
TGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCAAG229530.12912318868625916No Hit
TCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA227380.12791369600262106RNA PCR Primer, Index 1 (100% over 23bp)
TGGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGG222220.1250109135618896Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA219840.123672033288839RNA PCR Primer, Index 1 (100% over 22bp)
TGGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGA218150.12272131578402579RNA PCR Primer, Index 1 (100% over 22bp)
TTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAAC217270.12222626761583902RNA PCR Primer, Index 1 (100% over 24bp)
AAATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGGTGCCAAGG209280.11773145527059782Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG199360.11215091228376521No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGA196030.11027760501096757RNA PCR Primer, Index 1 (100% over 22bp)
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGA195330.10988381669536447RNA PCR Primer, Index 1 (100% over 22bp)
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA186170.1047308153083295RNA PCR Primer, Index 1 (100% over 23bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGC183640.10330755182479255No Hit
TGTTAATAGATAATATACGGATCTTAATATGGAATTCTCGGGTGCCAAGG178190.10024162851045405Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)