FastQCFastQC Report
Thu 29 Jun 2023
SRR1585929.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585929.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16542510
Total Bases827.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG2755751.6658596549133111No Hit
TTTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTC1099210.6644759471204793RNA PCR Primer, Index 1 (100% over 26bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAG947840.5729722998505064No Hit
TCAGATTTAGGCTCTGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTC894860.5409457210544228RNA PCR Primer, Index 1 (100% over 31bp)
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA850040.5138518882563771RNA PCR Primer, Index 1 (100% over 23bp)
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGGA849050.5132534301022034RNA PCR Primer, Index 1 (100% over 22bp)
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGGA772300.4668578105740906RNA PCR Primer, Index 1 (100% over 22bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG766510.4633577371269535Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA588030.3554660084835977RNA PCR Primer, Index 1 (100% over 22bp)
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT566880.34268076609897774RNA PCR Primer, Index 1 (100% over 25bp)
GGCGCCAGTGAAATACCACTACCTTGGAATTCTCGGGTGCCAAGGAACTC564130.34101838233738413RNA PCR Primer, Index 1 (100% over 26bp)
CCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAG537870.32514412867213016No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAGG491930.29737325230572625Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCCAAGG467040.28232716800533897Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGG392490.23726145548650115Illumina Small RNA Adapter 2 (100% over 21bp)
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC355100.21465908136068831RNA PCR Primer, Index 1 (100% over 24bp)
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGA336080.20316143076232082RNA PCR Primer, Index 1 (100% over 22bp)
TCACGTTCTAGCATTCAAGGTCCCGTGGAATTCTCGGGTGCCAAGGAACT330230.1996250871240217RNA PCR Primer, Index 1 (100% over 25bp)
TAGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGA329090.19893595349194287RNA PCR Primer, Index 1 (100% over 22bp)
TGGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGA325290.1966388413850135RNA PCR Primer, Index 1 (100% over 22bp)
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA323090.19530893437573862RNA PCR Primer, Index 1 (100% over 23bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG319580.19318712819275916No Hit
TGGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGG298800.18062555198697175Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGAA277270.16761059839165882RNA PCR Primer, Index 1 (100% over 23bp)
ACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTGCCAAGGAACT244620.14787356936764737RNA PCR Primer, Index 1 (100% over 25bp)
TCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA243700.14731742643649604RNA PCR Primer, Index 1 (100% over 23bp)
TCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAACTC243700.14731742643649604RNA PCR Primer, Index 1 (100% over 26bp)
GCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGGTGCCAAGGAA238960.14445208133469467RNA PCR Primer, Index 1 (100% over 23bp)
GGCGCCAGTGAAATACCACTACCTTTTGGAATTCTCGGGTGCCAAGGAAC237950.14384153311680029RNA PCR Primer, Index 1 (100% over 24bp)
TCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAACT226810.13710736762438108RNA PCR Primer, Index 1 (100% over 25bp)
TTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAAC215990.13056664315149275RNA PCR Primer, Index 1 (100% over 24bp)
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGA215160.13006490550708447RNA PCR Primer, Index 1 (100% over 22bp)
TGGCGCCAGTGAAATACCACTACCTTGGAATTCTCGGGTGCCAAGGAACT206950.12510193434974498RNA PCR Primer, Index 1 (100% over 25bp)
TTGTGGCGTCGCTGAACCATAGCAGTGGAATTCTCGGGTGCCAAGGAACT203010.12272019179677086RNA PCR Primer, Index 1 (100% over 25bp)
AAATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGGTGCCAAGG202300.12229099453468668Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCGGGTGCCAAGG201010.12151118542470278Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGTGCCAAGGAAC187570.11338666260440526RNA PCR Primer, Index 1 (100% over 24bp)
TTTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACT181050.1094453018314633RNA PCR Primer, Index 1 (100% over 25bp)
CCCCACTAATAGGGAACGTGAGCTGGGTTTGGAATTCTCGGGTGCCAAGG179730.10864735762589836Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAAC177040.10702124405546679RNA PCR Primer, Index 1 (100% over 24bp)
CGCGCGCTACACTGACGGAGCCAGCGTGGAATTCTCGGGTGCCAAGGAAC176750.10684593813151692RNA PCR Primer, Index 1 (100% over 24bp)
AGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAAC176510.10670085736686875RNA PCR Primer, Index 1 (100% over 24bp)
TTGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA172570.10431911481389461RNA PCR Primer, Index 1 (100% over 22bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTGGAATTCTCGGGTGCCAAGGA169220.10229402914068057RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAACT166170.10045029442327674RNA PCR Primer, Index 1 (100% over 25bp)
GGCGCCAGTGAAATACCACTACCTGGAATTCTCGGGTGCCAAGGAACTCC166070.10038984410467335RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)