FastQCFastQC Report
Thu 29 Jun 2023
SRR1585930.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585930.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19365429
Total Bases774.6 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG5836063.0136487035737756No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCG5786922.9882735879489166No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGG4364742.25388242109173No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGG2430931.2552936472515015No Hit
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGG1834140.9471207686646136No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGG1588170.8201057668280936No Hit
GCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGG1506160.7777571052001998No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCG1268870.6552243175196376No Hit
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGT1236290.6384005229112146No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGG1228800.6345328058572831No Hit
ATATAAAGAATGGTACAGCAATTAATATATGGAATTCTCG1228480.6343675629390911No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCG1191050.6150393053518205No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCG1163520.600823250546115No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGG1057610.5461330084657562No Hit
GCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGT896370.46287123306176176No Hit
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGT796110.4110985612557305No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGG795420.410742256213379No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTC740930.3826044855499974No Hit
TGTTAATAGATAATATACGGATCTTAATATGGAATTCTCG600810.310248742746675No Hit
AGCGGAGAATTTGGACAAACTTGGTCTGGAATTCTCGGGT577180.29804658600643447No Hit
AATACTGAATATCTAAATATTATTATTACTTGGAATTCTC539280.27847562788306934No Hit
CTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGG531870.2746492215586858No Hit
TTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGG483860.24985761998869224No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTC417520.21560069751101305No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCT406490.20990498067458252No Hit
TTTTAAAACCTAAAGGTAAACCTTTATTGGAATTCTCGGG400150.20663110535790352No Hit
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGG378150.19527065473220345No Hit
AAATCAGTTATCGTTTATTTGATAGTTTGGAATTCTCGGG366610.18931158199490444No Hit
AATGGCTTAGTGAGGCCTCAGGATCTTGGAATTCTCGGGT360730.1862752433731264No Hit
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGG354870.1832492324337354No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGTGGAATTCTCGGG352210.1818756506762644No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATT329400.17009692891389083No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGG327340.16903317762802983No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCG323800.16720517784553082No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC320440.16547012720451482No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGG309890.16002227474537228No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCTGTA296850.15328862582904826RNA PCR Primer, Index 11 (97% over 37bp)
TTGCTCGAATATATTAGCATGGAATATGGAATTCTCGGGT294800.15223003838438076No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGGAATTCTCGGGTG285550.14745348528039323No Hit
CGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGG285320.14733471693294273No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTGGAATTCTCGG280100.1446391918299357No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGG251980.13011847039381363No Hit
AATACTGAATATCTAAATATTATTATTACTGGAATTCTCG236930.12234688939759608No Hit
GGGAATACTGCCAGCTGGGACTGAGGATGGAATTCTCGGG225290.11633617824836208No Hit
ATATAAAGAATGGTACAGCAATTAATATATTGGAATTCTC224040.11569069809917457No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTGGAATTCTCGG221710.11448752310108906No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCG209940.10840968201633952No Hit
GTCAATTAAAATGTTACGTGAGTTGGGTTGGAATTCTCGG194540.10045736657834949No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)