FastQCFastQC Report
Thu 29 Jun 2023
SRR1585931.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585931.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30537007
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG9792433.20674190499416No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCG7389052.4197034110120876No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGG5520761.80789165094012No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGG3590521.1757930304040602No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATT2554520.8365325390271547No Hit
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGG2280840.7469101343166997No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCG2187580.7163701406624428No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGG1862200.6098174585348197No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGG1736330.5685986187185927No Hit
GCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGG1675290.5486097573347644No Hit
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGT1673230.5479351660102119No Hit
ATATAAAGAATGGTACAGCAATTAATATATGGAATTCTCG1572910.515083223447537No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCG1503300.4922879311649632No Hit
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGT1481870.48527021656051617No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCG1321470.43274378527011503No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGG1266980.41489986232115017No Hit
GCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGT1091010.3572746995145923No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTC1040020.34057692687433316No Hit
TGTTAATAGATAATATACGGATCTTAATATGGAATTCTCG1034700.3388347784051004No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGG857760.2808919682272726No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGG797200.26106029317149515No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCT763340.24997210761355887No Hit
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGG701850.2298358840471825No Hit
AGCGGAGAATTTGGACAAACTTGGTCTGGAATTCTCGGGT696580.22811010915378838No Hit
AATACTGAATATCTAAATATTATTATTACTTGGAATTCTC694770.22751738570842914No Hit
TTTTAAAACCTAAAGGTAAACCTTTATTGGAATTCTCGGG677510.22186522732892586No Hit
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGG635400.20807540175761166No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTC580300.19003172118341524No Hit
CTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGG572100.18734645474587605No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATT554100.1814519674439607No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATT514040.16833345848203132No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC505090.16540258840691233No Hit
TTGCTCGAATATATTAGCATGGAATATGGAATTCTCGGGT471060.15425873269112456No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTC470200.1539771071866997No Hit
TTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGG462300.15139008220419245No Hit
AAATCAGTTATCGTTTATTTGATAGTTTGGAATTCTCGGG390240.12779248470552468No Hit
GGGAATACTGCCAGCTGGGACTGAGGATGGAATTCTCGGG386900.12669872983950262No Hit
CGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGG382910.12539211848757803No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGTGGAATTCTCGGG370530.12133802110992738No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCG362670.118764094988091No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTGGAATTCTCGG344710.1128827065468466No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTC343600.11251921316322847No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGG342220.11206730247008162No Hit
TTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTG338040.11069847152997017No Hit
GTCAATTAAAATGTTACGTGAGTTGGGTTGGAATTCTCGG328730.10764971170881285No Hit
ATATAAAGAATGGTACAGCAATTAATATATTGGAATTCTC328240.10748925066559406No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTGGAATTCTCGG316080.10350719702163347No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCG310860.10179779570407801No Hit
TAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC308180.10092017203912615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)