FastQCFastQC Report
Wed 28 Jun 2023
SRR1585934.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585934.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18798567
Total Bases751.9 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG6144013.2683395494986405No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGG5708343.0365825224869534No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCG4984102.6513191138452203No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGG3124511.6621000951827871No Hit
GCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGG2687171.4294547025845108No Hit
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGG2257061.2006553478251827No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGG1780940.9473807232221477No Hit
GCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGT1351060.7187037182142659No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGG1284440.6832648467300725No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCG1203270.6400860235782866No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGG1174590.6248295415283516No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCG1164720.6195791413249744No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGG901460.47953655190845135No Hit
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGT870220.4629182639293729No Hit
CTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGG803640.4275006706628223No Hit
AGCGGAGAATTTGGACAAACTTGGTCTGGAATTCTCGGGT693630.3689802525905299No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGG691800.3680067741333688No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGG670340.35659101036797114No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCG655950.3489361715709501No Hit
ATATAAAGAATGGTACAGCAATTAATATATGGAATTCTCG593040.3154708547731324No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTC569310.3028475521565021No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCG568110.30220920562721615No Hit
GGGAATACTGCCAGCTGGGACTGAGGATGGAATTCTCGGG438990.23352311907604448No Hit
CGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGG416950.22179882115482527No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCT415680.22112323774466425No Hit
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGT396040.21067563288201702No Hit
ATGATTAATAGGGACGGTCGGGGGCATTGGAATTCTCGGG373240.19854704882558333No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGTGGAATTCTCGGG369950.19679691542445762No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATT367510.19549894414824276No Hit
AATGGCTTAGTGAGGCCTCAGGATCTTGGAATTCTCGGGT345730.1839129546417022No Hit
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGG337350.17945516804552178No Hit
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGG310800.1653317510850694No Hit
TGTTAATAGATAATATACGGATCTTAATATGGAATTCTCG295580.15723538927195888No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTGGAATTCTCGG295100.15698005066024448No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGG293550.15615551972658342No Hit
ATGATTAATAGGGACGGTCGGGGGCATGGAATTCTCGGGT291040.15482031156949358No Hit
TTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGG278040.1479048908355621No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTC275860.1467452279740259No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGGAATTCTCGGGTG260340.13848927952859386No Hit
GCCAAGGACGTTTTCATTAATCAAGAACTGGAATTCTCGG259090.1378243352272543No Hit
TCAGGGAGGTAGTGACAATAAATAACGTGGAATTCTCGGG254230.13523903178364607No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTC249310.13262181101357354No Hit
ATGATTAATAGGGACGGTCGGGGGCTGGAATTCTCGGGTG246550.13115361399621578No Hit
TTGCTCGAATATATTAGCATGGAATATGGAATTCTCGGGT210490.11197130079117201No Hit
CAGGGAGGTAGTGACAATAAATAACGTGGAATTCTCGGGT210430.11193938346470772No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCG205160.10913597829026012No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC202430.1076837399361345No Hit
GCGGAGAATTTGGACAAACTTGGTCTGGAATTCTCGGGTG196500.1045292441705796No Hit
TTTTAAAACCTAAAGGTAAACCTTTATTGGAATTCTCGGG194150.10327914888406123No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACGGTGA192460.10238014418865013RNA PCR Primer, Index 5 (97% over 40bp)
TTGATGATTCATAATAACTTTTCGAATCGCTGGAATTCTC192310.10230035087248938No Hit
AAATCAGTTATCGTTTATTTGATAGTTTGGAATTCTCGGG191790.10202373404313211No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCG190950.10157689147263192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)