FastQCFastQC Report
Thu 29 Jun 2023
SRR1585935.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585935.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25949659
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGG8685813.3471769320745213No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCG7728032.9780853767673787No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCG7178422.7662868325167587No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGG4808011.8528220351566083No Hit
GCGGAGAATTTGGACAAACTTGGTCATTGGAATTCTCGGG3200021.233164566825329No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGG2679651.0326339933792579No Hit
AGCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGG2490140.9596041319849329No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGG1643870.6334842396194879No Hit
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGT1554390.5990020909330639No Hit
GCGGAGAATTTGGACAAACTTGGTCATGGAATTCTCGGGT1517360.5847321538984385No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGG1398030.5387469638811053No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCG1297420.49997574149240265No Hit
CTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGG1160170.44708487306133776No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCG1031410.39746572392338564No Hit
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGT978200.37696063751743325No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCG960090.3699817404151631No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGG957580.36901448300341827No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGG871040.3356652971817472No Hit
ATATAAAGAATGGTACAGCAATTAATATATGGAATTCTCG777720.2997033602638092No Hit
AGCGGAGAATTTGGACAAACTTGGTCTGGAATTCTCGGGT771870.2974489953798622No Hit
CGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGG642450.24757550763961866No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATT631440.24333267731957478No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGTGGAATTCTCGGG621910.23966018204709355No Hit
TGTTAATAGATAATATACGGATCTTAATATGGAATTCTCG552900.21306638364689107No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGG540370.2082378038185396No Hit
AATGGCTTAGTGAGGCCTCAGGATCTTGGAATTCTCGGGT538050.20734376509533325No Hit
GGGAATACTGCCAGCTGGGACTGAGGATGGAATTCTCGGG537510.2071356698752766No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCG495630.1909967294753276No Hit
TTTTAAAACCTAAAGGTAAACCTTTATTGGAATTCTCGGG488880.1883955392246195No Hit
TTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGG472320.18201395247621557No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTGGAATTCTCGG472110.18193302655730467No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGG439520.16937409466536726No Hit
TTGCTCGAATATATTAGCATGGAATATGGAATTCTCGGGT432370.16661875980720978No Hit
GCCAAGGACGTTTTCATTAATCAAGAACTGGAATTCTCGG428820.16525072641609664No Hit
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGG423860.16333933328372446No Hit
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGG363480.14007120478924212No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTC346970.1337088861167694No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTGGAATTCTCGG336830.12980132031792788No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGGAATTCTCGGGTG324620.12509605617553587No Hit
TAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTC299640.11546972544032273No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTC295450.11385506067729059No Hit
AATACTGAATATCTAAATATTATTATTACTTGGAATTCTC289930.1117278650944893No Hit
ATGATTAATAGGGACGGTCGGGGGCATTGGAATTCTCGGG285180.10989739788102802No Hit
CAGGGAGGTAGTGACAATAAATAACGTGGAATTCTCGGGT267900.10323835083921526No Hit
AAATCAGTTATCGTTTATTTGATAGTTTGGAATTCTCGGG262170.10103022933750305No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCG261890.10092232811228848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)