FastQCFastQC Report
Thu 29 Jun 2023
SRR1585938.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585938.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26427516
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length80
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGA6793432.5705896838734295RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGGGCGAAATCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1940410.7342385111033516RNA PCR Primer, Index 1 (100% over 32bp)
TCCCGCTAGAGACCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA799430.3024991073697581RNA PCR Primer, Index 1 (100% over 32bp)
TCCACAGAATTCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA667010.2523922414804327RNA PCR Primer, Index 1 (100% over 32bp)
ATCCTGTCTGTGACGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA627810.2375592166891508RNA PCR Primer, Index 1 (100% over 32bp)
CGGTTCGATTCCGGGCTTGCGCACCATGGAATTCTCGGGTGCCAAGGAAC595480.22532575517123896RNA PCR Primer, Index 1 (100% over 24bp)
CTCGCCCCCGGGAGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA563970.21340257631477735RNA PCR Primer, Index 1 (100% over 32bp)
TCCTGAGTGCGAGCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA541100.20474871720823098RNA PCR Primer, Index 1 (100% over 32bp)
ATCCGATTGGAAGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA502040.18996866750548935RNA PCR Primer, Index 1 (100% over 32bp)
ACCTCCCCTGGAGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA472440.1787682202141132RNA PCR Primer, Index 1 (100% over 32bp)
CCCTCACTGGGGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA463020.17520375354233064RNA PCR Primer, Index 1 (100% over 32bp)
AGATCGGGGTTCAATTCCCCGTCGCGGAGCCATGGAATTCTCGGGTGCCA458500.17349341496945833No Hit
CCCCCCATCGTGAGTGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTC425350.1609496707900962RNA PCR Primer, Index 1 (100% over 31bp)
AGGTTCGAGTCCTGCAGTTGTCGCCATGGAATTCTCGGGTGCCAAGGAAC389270.14729723368628364RNA PCR Primer, Index 1 (100% over 24bp)
ATCCGGGTAGGACCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA387980.14680910608473383RNA PCR Primer, Index 1 (100% over 32bp)
TTCCTGTCCGTTTCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA385110.1457231167696578RNA PCR Primer, Index 1 (100% over 32bp)
ATCGGGGTTCAATTCCCCGTCGCGGAGCCATGGAATTCTCGGGTGCCAAG382370.14468631860822637No Hit
ACCTCGGTTGGATCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA377080.14268461704838245RNA PCR Primer, Index 1 (100% over 32bp)
GGTTCGATTCCGGGCTTGCGCACCATGGAATTCTCGGGTGCCAAGGAACT367240.13896122511097903RNA PCR Primer, Index 1 (100% over 25bp)
GCCCCCTATGAGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA361730.13687627698342894RNA PCR Primer, Index 1 (100% over 32bp)
TCGGGGTTCAATTCCCCGTCGCGGAGCCATGGAATTCTCGGGTGCCAAGG332500.12581583528319498Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTAGTGGTAAAATCCAACGTTGCTGGAATTCTCGGGTGCCAAGGAACT329660.12474119777280618RNA PCR Primer, Index 1 (100% over 25bp)
GTCTAGTCGGTTATGGCATCTGCTGGAATTCTCGGGTGCCAAGGAACTCC299320.11326073929914564RNA PCR Primer, Index 1 (100% over 27bp)
GCCCAGTTGGTTAAGGCACCGTGCTGGAATTCTCGGGTGCCAAGGAACTC297230.11246989690593698RNA PCR Primer, Index 1 (100% over 26bp)
GTCTAGTCGGTTATGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC278220.10527663666914439RNA PCR Primer, Index 1 (100% over 33bp)
TGGGAGAGGTCTCCGGTTCGATGGAATTCTCGGGTGCCAAGGAACTCCAG277470.10499284155196424RNA PCR Primer, Index 1 (100% over 29bp)
GTTTAGTGGTAAAATCCAACTGGAATTCTCGGGTGCCAAGGAACTCCAGT276730.10471283036967985RNA PCR Primer, Index 1 (100% over 30bp)
GTTTAGTGGTAAAATCCAACGTTTGGAATTCTCGGGTGCCAAGGAACTCC271480.10272626454941886RNA PCR Primer, Index 1 (100% over 27bp)
GTCTAGTGGTATGATTCTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCC265560.10048617509114365RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)