FastQCFastQC Report
Thu 29 Jun 2023
SRR1585939.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585939.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22403109
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length80
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGA461792320.612866723096335RNA PCR Primer, Index 1 (100% over 22bp)
TGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAA1778420.7938273210204887RNA PCR Primer, Index 1 (100% over 23bp)
TACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACT950980.42448572651233357RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAACCCATCTCGTATGC813880.36328886316626857RNA PCR Primer, Index 9 (97% over 37bp)
ATGTACACGGAGTCGACCCAACGCGTGGAATTCTCGGGTGCCAAGGAACT800200.3571825678302061RNA PCR Primer, Index 1 (100% over 25bp)
TCCTGGGCGAAATCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA702720.3136707498945794RNA PCR Primer, Index 1 (100% over 32bp)
ACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTC608020.271399831157363RNA PCR Primer, Index 1 (100% over 26bp)
TATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGG543760.24271631227612203Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTCCA524300.2340300178872495RNA PCR Primer, Index 1 (100% over 28bp)
GAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC360210.1607857195177687RNA PCR Primer, Index 1 (100% over 31bp)
ATGTACACGGAGTCGACCCGCAACGCGTGGAATTCTCGGGTGCCAAGGAA350350.15638454466297513RNA PCR Primer, Index 1 (100% over 23bp)
CACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTCC331330.1478946515860812RNA PCR Primer, Index 1 (100% over 27bp)
CGGTTCGATTCCGGGCTTGCGCACCATGGAATTCTCGGGTGCCAAGGAAC331010.14775181426827858RNA PCR Primer, Index 1 (100% over 24bp)
TCCCGCTAGAGACCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA327070.14599312979283366RNA PCR Primer, Index 1 (100% over 32bp)
AGATCGGGGTTCAATTCCCCGTCGCGGAGCCATGGAATTCTCGGGTGCCA278700.12440237647372959No Hit
ATGTACACGGAGTCGACCCGCAACTGGAATTCTCGGGTGCCAAGGAACTC263340.11754618521920329RNA PCR Primer, Index 1 (100% over 26bp)
TCCACAGAATTCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA254780.11372528696798288RNA PCR Primer, Index 1 (100% over 32bp)
GGAATTCTCGGGTGCCAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT243860.10885096349796806RNA PCR Primer, Index 1 (100% over 30bp)
TCGGGGTTCAATTCCCCGTCGCGGAGCCATGGAATTCTCGGGTGCCAAGG236850.10572193350485418Illumina Small RNA Adapter 2 (100% over 21bp)
GGTTCGATTCCGGGCTTGCGCACCATGGAATTCTCGGGTGCCAAGGAACT234060.10447657064026247RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)