FastQCFastQC Report
Thu 29 Jun 2023
SRR1585940.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585940.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26305808
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length80
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGA17368416.602500101878642RNA PCR Primer, Index 1 (100% over 22bp)
ATGTACACGGAGTCGACCCAACGCGTGGAATTCTCGGGTGCCAAGGAACT1720130.6538974206760728RNA PCR Primer, Index 1 (100% over 25bp)
TACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACT1250140.4752334541482246RNA PCR Primer, Index 1 (100% over 25bp)
TGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAA1096190.4167102565334621RNA PCR Primer, Index 1 (100% over 23bp)
ACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTCCA1029220.3912520003187129RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGGGCGAAATCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA905330.3441559369702691RNA PCR Primer, Index 1 (100% over 32bp)
ACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTC669610.254548349170647RNA PCR Primer, Index 1 (100% over 26bp)
CACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTCC633730.2409087757350012RNA PCR Primer, Index 1 (100% over 27bp)
CGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTCCAG610480.2320704233833076RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCTGAGATCTCGTATGC484620.18422547598613964RNA PCR Primer, Index 25 (97% over 38bp)
GAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC465710.17703694940676218RNA PCR Primer, Index 1 (100% over 31bp)
CGGTTCGATTCCGGGCTTGCGCACCATGGAATTCTCGGGTGCCAAGGAAC434600.1652106637439154RNA PCR Primer, Index 1 (100% over 24bp)
TCCCGCTAGAGACCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA385850.1466786346193966RNA PCR Primer, Index 1 (100% over 32bp)
AGATCGGGGTTCAATTCCCCGTCGCGGAGCCATGGAATTCTCGGGTGCCA343080.1304198677341521No Hit
TCCACAGAATTCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA330820.1257592999994526RNA PCR Primer, Index 1 (100% over 32bp)
GGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGT327260.12440598669312877RNA PCR Primer, Index 1 (100% over 30bp)
AGGTTCGAGTCCTGCAGTTGTCGCCATGGAATTCTCGGGTGCCAAGGAAC314620.11960096416730481RNA PCR Primer, Index 1 (100% over 24bp)
TCACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTC306210.11640395155320833RNA PCR Primer, Index 1 (100% over 26bp)
TCGGGGTTCAATTCCCCGTCGCGGAGCCATGGAATTCTCGGGTGCCAAGG301330.11454884791982059Illumina Small RNA Adapter 2 (100% over 21bp)
AGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA271240.10311030932788684RNA PCR Primer, Index 1 (100% over 32bp)
GGTTCGATTCCGGGCTTGCGCACCATGGAATTCTCGGGTGCCAAGGAACT268300.10199268541760817RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)