FastQCFastQC Report
Thu 29 Jun 2023
SRR1585941.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1585941.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28047731
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length80
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGA7738512.7590502775429497RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGGGCGAAATCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1006070.35869924736514336RNA PCR Primer, Index 1 (100% over 32bp)
ACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTCCA851780.3036894499594281RNA PCR Primer, Index 1 (100% over 28bp)
TACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACT694180.24749952144078963RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTCAGAATCTCGTATGC645400.2301077402660486RNA PCR Primer, Index 40 (98% over 50bp)
ATGTACACGGAGTCGACCCAACGCGTGGAATTCTCGGGTGCCAAGGAACT616100.21966126243866216RNA PCR Primer, Index 1 (100% over 25bp)
TGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAA510110.18187210936956005RNA PCR Primer, Index 1 (100% over 23bp)
CACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTCC470020.16757861803509166RNA PCR Primer, Index 1 (100% over 27bp)
TCCACAGAATTCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA445990.15901108007631704RNA PCR Primer, Index 1 (100% over 32bp)
CGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTCCAG440960.15721770862676912RNA PCR Primer, Index 1 (100% over 29bp)
CGGTTCGATTCCGGGCTTGCGCACCATGGAATTCTCGGGTGCCAAGGAAC426610.1521014302369058RNA PCR Primer, Index 1 (100% over 24bp)
ACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGAACTC402130.1433734514923863RNA PCR Primer, Index 1 (100% over 26bp)
TCCCGCTAGAGACCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA402040.14334136333523734RNA PCR Primer, Index 1 (100% over 32bp)
AGATCGGGGTTCAATTCCCCGTCGCGGAGCCATGGAATTCTCGGGTGCCA371200.1323458214855241No Hit
AGGTTCGAGTCCTGCAGTTGTCGCCATGGAATTCTCGGGTGCCAAGGAAC358230.12772156150527827RNA PCR Primer, Index 1 (100% over 24bp)
ATCCGATTGGAAGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA319610.11395217673757638RNA PCR Primer, Index 1 (100% over 32bp)
CTCGCCCCCGGGAGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA310520.11071127286553055RNA PCR Primer, Index 1 (100% over 32bp)
ATCCTGTCTGTGACGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA292760.10437920985480074RNA PCR Primer, Index 1 (100% over 32bp)
TCGGGGTTCAATTCCCCGTCGCGGAGCCATGGAATTCTCGGGTGCCAAGG292040.104122504597609Illumina Small RNA Adapter 2 (100% over 21bp)
ATCGGGGTTCAATTCCCCGTCGCGGAGCCATGGAATTCTCGGGTGCCAAG289770.10331316996729611No Hit
GGAATTCTCGGGTGCCAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT286920.1022970449909121RNA PCR Primer, Index 1 (100% over 30bp)
GGTTCGATTCCGGGCTTGCGCACCATGGAATTCTCGGGTGCCAAGGAACT282340.10066411432710903RNA PCR Primer, Index 1 (100% over 25bp)
TCCTGAGTGCGAGCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA282070.10056784985566213RNA PCR Primer, Index 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)