FastQCFastQC Report
Tue 19 Sept 2023
SRR15882954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15882954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92454545
Total Bases13.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA86669229.374251963491897RNA PCR Primer, Index 1 (100% over 32bp)
GGGATTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA49538575.3581541069722425RNA PCR Primer, Index 1 (100% over 32bp)
TGGGTTTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC42142264.558159904415732RNA PCR Primer, Index 1 (100% over 31bp)
GGGGTTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA27444062.9683840853902854RNA PCR Primer, Index 1 (100% over 32bp)
GTTTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT21576962.3337911619163774RNA PCR Primer, Index 3 (100% over 34bp)
TGGGATTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC15728621.7012273436638512RNA PCR Primer, Index 1 (100% over 31bp)
GGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA15630771.6906437644574421RNA PCR Primer, Index 10 (100% over 35bp)
GGGTTTAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA12743981.3784049231976643RNA PCR Primer, Index 10 (100% over 35bp)
GATTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT12684721.3719952869812944RNA PCR Primer, Index 3 (100% over 34bp)
GGGTTTAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC12654621.3687396330813157RNA PCR Primer, Index 1 (100% over 33bp)
AGCCGGATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGA12652351.3684941070230783RNA PCR Primer, Index 42 (100% over 40bp)
GGTTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT12174831.316844942560693RNA PCR Primer, Index 3 (100% over 34bp)
TGGGGTTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC11006621.1904898780260071RNA PCR Primer, Index 1 (100% over 31bp)
GGGTTTAGACCGTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT8341340.9022098372773344RNA PCR Primer, Index 3 (100% over 34bp)
GGGATTAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC7685070.8312268477444781RNA PCR Primer, Index 1 (100% over 33bp)
TGGGTTTAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA7560650.8177694238828389RNA PCR Primer, Index 1 (100% over 32bp)
GGGTTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC7501160.8113349105768677RNA PCR Primer, Index 1 (100% over 33bp)
AGCCGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGAT6680480.7225691284295434RNA PCR Primer, Index 42 (100% over 41bp)
GGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCG6673500.7218141628407776RNA PCR Primer, Index 42 (100% over 39bp)
GGGATTAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA6454010.6980738480731261RNA PCR Primer, Index 10 (100% over 35bp)
TGGGTTTAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT6232890.6741572304530837RNA PCR Primer, Index 3 (100% over 34bp)
GACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCG5831970.630793218440478RNA PCR Primer, Index 42 (100% over 39bp)
TGGGGATCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGAT4916230.5317456269997326RNA PCR Primer, Index 42 (100% over 41bp)
GGGGTTAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4716650.5101588029014691RNA PCR Primer, Index 1 (100% over 33bp)
AGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATC4623830.5001192748285117RNA PCR Primer, Index 42 (100% over 38bp)
TTTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA4477080.48424661005037667RNA PCR Primer, Index 10 (100% over 35bp)
GTTTAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA4393760.4752346139392066RNA PCR Primer, Index 10 (100% over 35bp)
AAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGA4250480.4597372687302717RNA PCR Primer, Index 42 (100% over 40bp)
GGGATTAGACCGTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT4179700.4520816148086608RNA PCR Primer, Index 3 (100% over 34bp)
GGGGTTAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA3615980.39110894980879524RNA PCR Primer, Index 10 (100% over 35bp)
GCAGCTAACGCGTTAAATGATCCGCCTGAGTAGTGGAATTCTCGGGTGCC3280990.3548760096109932No Hit
TGGGTTTAGACCGTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3272440.3539512308453846RNA PCR Primer, Index 1 (100% over 33bp)
TGGGATTAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3111950.33659243036672776RNA PCR Primer, Index 1 (100% over 32bp)
AGAGGTGAAATTCTTGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3037450.32853441656113286RNA PCR Primer, Index 1 (100% over 30bp)
GTTTAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAT2966890.32090255811653173RNA PCR Primer, Index 42 (100% over 37bp)
AGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTC2903080.31400078817109534RNA PCR Primer, Index 42 (100% over 44bp)
CGGGGAGAGTTCTCTTTTCTTTGTGATGGAATTCTCGGGTGCCAAGGAAC2882980.31182674686247175RNA PCR Primer, Index 1 (100% over 24bp)
GAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATC2861630.3095175039799287RNA PCR Primer, Index 42 (100% over 38bp)
GGGTTTAGACCGTCGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2789670.30173421977253795RNA PCR Primer, Index 1 (100% over 31bp)
GGGATTAGACCGTCGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2614310.2827670613705362RNA PCR Primer, Index 1 (100% over 31bp)
CGGATAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGAT2275900.2461642096664907RNA PCR Primer, Index 42 (100% over 41bp)
TGGGATTAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT2231300.24134021750904727RNA PCR Primer, Index 3 (100% over 34bp)
GGTTAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA2222510.24038948004124622RNA PCR Primer, Index 10 (100% over 35bp)
GGGGTTAGACCGTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT2138820.231337464264196RNA PCR Primer, Index 3 (100% over 34bp)
TGGGGTTAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2098140.22693746424256372RNA PCR Primer, Index 1 (100% over 32bp)
AGCAGAACTGGCGCTGCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG2071690.22407659893842968RNA PCR Primer, Index 1 (100% over 29bp)
TGGGTTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2070950.22399655960666942RNA PCR Primer, Index 1 (100% over 32bp)
GATTAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA2054330.2221989194798374RNA PCR Primer, Index 10 (100% over 35bp)
GGCATGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGA1983300.2145162252434426RNA PCR Primer, Index 42 (100% over 40bp)
ATTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1943050.2101627345632386RNA PCR Primer, Index 10 (100% over 35bp)
TTTGGTGTATGTGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1927600.20849164310959512RNA PCR Primer, Index 1 (100% over 32bp)
TGGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTC1880490.20339616619172157RNA PCR Primer, Index 42 (100% over 44bp)
ACTCTGGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGAT1862920.2014957728687108RNA PCR Primer, Index 42 (100% over 41bp)
CCATGAGTTGGTAGTTTCGGTTGGGTGGAATTCTCGGGTGCCAAGGAACT1830760.198017306774913RNA PCR Primer, Index 1 (100% over 25bp)
TTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA1809690.19573834904492798RNA PCR Primer, Index 42 (100% over 36bp)
CGGCCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGAT1785350.19310570399756982RNA PCR Primer, Index 42 (100% over 41bp)
CATTTGGTGTATGTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1725480.18663008941312728RNA PCR Primer, Index 1 (100% over 33bp)
GTTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1688220.18260000089773845RNA PCR Primer, Index 10 (100% over 35bp)
TGGGGTTAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1683920.1821349074834558RNA PCR Primer, Index 3 (100% over 34bp)
GACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATC1672740.18092566460632087RNA PCR Primer, Index 42 (100% over 42bp)
CTGAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA1629790.17628013852645102RNA PCR Primer, Index 42 (100% over 36bp)
GACTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATC1615560.17474100380895283RNA PCR Primer, Index 42 (100% over 38bp)
GGGTTTAGACCGTCGTGAGATGGAATTCTCGGGTGCCAAGGAACTCCAGT1582870.17120521224781324RNA PCR Primer, Index 1 (100% over 30bp)
AGTAAGGGCATATGGTGAATGCCTTGGAAAATGGAGCTGGAATTCTCGGG1579560.17084719848007474No Hit
GCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA1563830.16914582187387328RNA PCR Primer, Index 42 (100% over 36bp)
TGAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAT1552520.16792251803305075RNA PCR Primer, Index 42 (100% over 37bp)
CCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCT1524300.16487020730024685RNA PCR Primer, Index 42 (100% over 43bp)
GGGTTAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1478530.1599196664696149RNA PCR Primer, Index 3 (100% over 34bp)
TCTGGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA1470200.15901868318101614RNA PCR Primer, Index 42 (100% over 36bp)
TGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTC1466260.15859252782002226RNA PCR Primer, Index 42 (100% over 44bp)
ATTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATC1462120.1581447402072013RNA PCR Primer, Index 42 (100% over 42bp)
TGGGTTTAGACCGTCGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1447150.1565255661579428RNA PCR Primer, Index 1 (100% over 30bp)
GGGGTTAGACCGTCGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1422500.15385939112025265RNA PCR Primer, Index 1 (100% over 31bp)
GGGTTTAGACCGTCGTGAGACATGGAATTCTCGGGTGCCAAGGAACTCCA1371280.14831937142733223RNA PCR Primer, Index 1 (100% over 28bp)
CCCTGAACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGAT1346370.14562507446226683RNA PCR Primer, Index 42 (100% over 41bp)
CGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTC1302580.14088869292472317RNA PCR Primer, Index 42 (100% over 44bp)
CGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCG1300750.1406907578205052RNA PCR Primer, Index 42 (100% over 45bp)
TCTGGCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGA1283730.13884985319001894RNA PCR Primer, Index 42 (100% over 40bp)
CTTCGGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGA1274250.1378244844534144RNA PCR Primer, Index 42 (100% over 40bp)
TGGGTTTAGACCGTCGTGAGATGGAATTCTCGGGTGCCAAGGAACTCCAG1260200.13630481876256056RNA PCR Primer, Index 1 (100% over 29bp)
CCCACTGTAGCTTTAAAAGTTAGTGGAATTCTCGGGTGCCAAGGAACTCC1247600.13494198689745324RNA PCR Primer, Index 1 (100% over 27bp)
GGGATTAGACCGTCGTGAGATGGAATTCTCGGGTGCCAAGGAACTCCAGT1245510.13471592986586003RNA PCR Primer, Index 1 (100% over 30bp)
TGCCGACTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGA1244090.13456234087788763RNA PCR Primer, Index 42 (100% over 40bp)
AAGCAGAACTGGCGCTGCGGGATGGAATTCTCGGGTGCCAAGGAACTCCA1216750.13160521205312295RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGTGTATGTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1178370.12745398292750237RNA PCR Primer, Index 10 (100% over 35bp)
GACTGAACGCCTCTAAGTCATGGAATTCTCGGGTGCCAAGGAACTCCAGT1173100.12688397309185828RNA PCR Primer, Index 1 (100% over 30bp)
GTTTAGACCGTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA1161300.12560767023405936RNA PCR Primer, Index 42 (100% over 36bp)
GATTAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAT1150490.12443844702280456RNA PCR Primer, Index 42 (100% over 37bp)
ACATTTGGTGTATGTGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG1131180.12234985310889801RNA PCR Primer, Index 1 (100% over 29bp)
TCTCGTCTGATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAT1131140.12234552665853259RNA PCR Primer, Index 42 (100% over 37bp)
CTGGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAT1126500.12184365841614385RNA PCR Primer, Index 42 (100% over 37bp)
GGTTAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAT1126250.12181661810135996RNA PCR Primer, Index 42 (100% over 37bp)
TGACTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAT1117420.1208615541831935RNA PCR Primer, Index 42 (100% over 37bp)
CGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTC1069160.11564169181731412RNA PCR Primer, Index 42 (100% over 44bp)
GCTAGAGGTGAAATTCTTGGACCTGGAATTCTCGGGTGCCAAGGAACTCC1058290.1144659789305112RNA PCR Primer, Index 1 (100% over 27bp)
GCAGCTAACGCGTTAAATGATCCGCCTGAGTATGGAATTCTCGGGTGCCA1050450.11361799465888886No Hit
CGGTTGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGA1032400.11166568393149304RNA PCR Primer, Index 42 (100% over 40bp)
TAGTTGGATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCG1004610.10865988254011741RNA PCR Primer, Index 42 (100% over 39bp)
GGGTTAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA988910.10696175077169001RNA PCR Primer, Index 42 (100% over 36bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA987970.10686007918810266RNA PCR Primer, Index 1 (100% over 28bp)
GGGTCGGGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG972640.1052019670855554RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGC964730.10434641152579356RNA PCR Primer, Index 42 (100% over 50bp)
TGTATGTGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAT955130.10330806343809273RNA PCR Primer, Index 42 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph