FastQCFastQC Report
Sat 16 Sept 2023
SRR15906252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19406417
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG11221605.782417228280727No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA6761453.484131047993043No Hit
CGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGA2253791.1613632748384208No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA2025131.0435362694721029No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA1683150.8673162078296061No Hit
TCGACGTGGGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1664020.8574586437053269No Hit
TTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAA1442620.743372668947596No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA1398830.7208079677974558No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC1373370.707688595993789No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG1337970.6894472070758862No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT1328370.6845003897422177No Hit
ACACGAGAGCTTATGCCCTATGTATGGCCTGTAGGCACCATCAATAGATC1175450.6057017119646558No Hit
TCGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGA1135940.5853424668757762No Hit
TCGACTCTTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAA967000.4982887876726549No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA787880.4059894209219559Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGAAGAGCACACG778390.40109928587023563No Hit
TTTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG748240.38556318768168285No Hit
TGTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG746020.38441923617327195No Hit
ACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT725040.3736083791253172No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT723220.3726705450058092No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCAT647410.33360614687399537No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG627750.32347547720941994No Hit
TTGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA622040.3205331514828317No Hit
TCTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG567700.2925321042003787No Hit
ATGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA560320.288729238375121No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG524660.2703538731544314No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG519890.26789592329176476No Hit
TTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG474320.2444139997610069No Hit
ACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG473680.24408421193876229Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAG469970.2421724731566883No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA467750.24102852164827748No Hit
ATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC451050.23242312066158324No Hit
ACACGAGAGCTTATGCCCTATGTATGGCCCTGTAGGCACCATCAATAGAT441130.2273114094167924No Hit
TTGGGTTCGTGGCGGCTAGCGTTTCTGTAGGCACCATCAATAGATCGGAA429860.22150405198445444No Hit
AACACGAGAGCTTATGCCCTACTGTAGGCACCATCAATAGATCGGAAGAG411360.21197112274769733No Hit
ATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT393520.20277828720262994No Hit
TTGGGTTCGTGGCGGCTAGCCTGTAGGCACCATCAATAGATCGGAAGAGC390310.20112419515668453No Hit
ACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA385490.19864048062040507No Hit
TTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAAGAGCACA383290.1975068349814394No Hit
TGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGAG382920.19731617639670426No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACC372760.19208079471857167No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC360170.18559324990285428No Hit
ATTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG343250.1768744843522635No Hit
ACACGAGAGCTTATGCCCTACTGTAGGCACCATCAATAGATCGGAAGAGC340220.17531314513132434No Hit
TCTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCG338540.17444745209793236No Hit
TTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGAAGAGCAC335090.1726696896186452No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA334450.17233990179640063No Hit
TTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG315090.1623638201735024No Hit
TCGGGAGGCATCTCTATCTCGTGGCTGTAGGCACCATCAATAGATCGGAA312520.16103951594980156No Hit
TTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA307130.15826208413433557No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA298700.15391816016320786No Hit
TGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGA298080.15359867821040846No Hit
GTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC288670.14874976663646874No Hit
TTGGGTTCGTGGCGGCTAGCGTTCTGTAGGCACCATCAATAGATCGGAAG288600.14871369609341076No Hit
TTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATA288560.14869308435452047No Hit
ACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGATC285640.14718842741552962No Hit
AAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAA272530.1404329299942385No Hit
TTTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAAGAGCAC271100.1396960603289108No Hit
TATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT261110.13454827854106197No Hit
CTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG261100.13454312560633938No Hit
TGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCA257430.13265199856315568No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACAATCAATAGATCGGAAG257060.13246133997842055No Hit
TGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC256590.13221915204645968No Hit
AACACGAGAGCTTATGCCCTATCTGTAGGCACCATCAATAGATCGGAAGA246390.12696315862943686No Hit
CCGACGTGGGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC245670.1265921473294117No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT244000.12573160723074228No Hit
TTGACGACAGTGAAATACCACCACTCTGTAGGCACCATCAATAGATCGGA243550.12549972516822655No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA240760.12406205638062916No Hit
ACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT235930.12157318890962718No Hit
TTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGAAGAGCA235030.12110942478459573No Hit
TCGAGTACAGACCGCGAAAGCGTGGCCTATCGCTGTAGGCACCATCAATA228390.11768787612880832No Hit
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCA227190.11706952396209976No Hit
AGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT223660.11525053800503204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAGAG218590.11263800010068833No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA217790.11222576532288263No Hit
AAGGAGAGGGCAAGTCTGGTCTGTAGGCACCATCAATAGATCGGAAGAGC216280.11144767217977435No Hit
TCTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATC212970.10974205078660322No Hit
ACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT203030.10462003367236723Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGG202520.1043572340015161No Hit
TGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAG197860.10195596642079782No Hit
GGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGAG197010.10151796696937926No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)