FastQCFastQC Report
Sat 16 Sept 2023
SRR15906253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19467837
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG14602117.500632967083091No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA4434502.2778596307335017No Hit
CGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGA3060221.5719363173217447No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC1660070.8527244192562327No Hit
ACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1578880.81101973475533No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG1518180.7798401024212397No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA1491650.7662124970534734No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA1396260.7172137305238379No Hit
ACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1356190.6966310638413502No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA1319130.6775945370818546No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1305730.6707113892519235Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT1123350.5770286652800719No Hit
TCGACTCTTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAA1101900.5660104920746973No Hit
TTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAA1065860.547497906418674No Hit
TCGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGA974800.5007233212400536No Hit
ACACGAGAGCTTATGCCCTATGTATGGCCTGTAGGCACCATCAATAGATC928400.4768891377095462No Hit
ATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC812820.417519419337649No Hit
TTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAAGAGCACA783630.4025254577588666No Hit
ATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT764770.39283768402211294No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG732620.37632326590776366No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCAT697670.35837057809760786No Hit
TTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG563130.2892617192141068No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT560380.2878491329057255No Hit
GTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC559380.2873354651572232No Hit
TTGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA555610.28539893774536945No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG511950.26297220384575853No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC511530.2627564633913876No Hit
TTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGAAGAGCAC477240.24514279629524327No Hit
TTGGGTTCGTGGCGGCTAGCGTTTCTGTAGGCACCATCAATAGATCGGAA467930.24036054955668676No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA456000.2342324933170542No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA454140.23327707130483985No Hit
ACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG442480.22728770535730292Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA435510.2237074411502418No Hit
TTGGGTTCGTGGCGGCTAGCCTGTAGGCACCATCAATAGATCGGAAGAGC414290.21280741152702276No Hit
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAG412030.21164652241540752No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG410280.2107476038555285No Hit
ATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC392160.2014399442526666No Hit
TTTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAAGAGCAC389490.20006845136416543No Hit
TGTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG371780.19097139553818948No Hit
ACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGATC366340.1881770429863369No Hit
TATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT358730.18426803142023432No Hit
TTTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG358500.18414988783807879No Hit
TCGGGAGGCATCTCTATCTCGTGGCTGTAGGCACCATCAATAGATCGGAA356140.1829376319516133No Hit
ACACGAGAGCTTATGCCCTATGTATGGCCCTGTAGGCACCATCAATAGAT355270.1824907410104163No Hit
TCGACGTGGGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC341850.1755973198255153No Hit
AACACGAGAGCTTATGCCCTACTGTAGGCACCATCAATAGATCGGAAGAG337750.1734912820566558No Hit
ATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT331370.17021408182121106No Hit
TTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATA312660.1606033582467328No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA306820.1576035385954793No Hit
TTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA306540.15745971162589867No Hit
ACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA300770.1544958487170403No Hit
AACACGAGAGCTTATGCCCTATCTGTAGGCACCATCAATAGATCGGAAGA299810.15400272767847809No Hit
TGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGAG293700.15086421773512895No Hit
AAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAA288120.14799795169848606No Hit
TTGACGACAGTGAAATACCACCACTCTGTAGGCACCATCAATAGATCGGA288080.14797740498854597No Hit
TTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG287600.14773084446926488No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACC284020.14589191392962658No Hit
ACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT280750.14421222039202403No Hit
ACACGAGAGCTTATGCCCTACTGTAGGCACCATCAATAGATCGGAAGAGC279390.14351363225406089No Hit
TGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGA272450.13994877807945483No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACAATCAATAGATCGGAAG269990.13868515541813917No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA267820.13757049640388913No Hit
TCTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG267680.13749858291909883No Hit
TGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCA260400.13375908171000198No Hit
TCGAGTACAGACCGCGAAAGCGTGGCCTATCGCTGTAGGCACCATCAATA256950.131986927977669No Hit
CTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG250350.12859672083755375No Hit
TACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAAGAGCACACG249770.1282987935434224No Hit
TGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC248160.12747178846833368No Hit
TTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGAAGAGCA238540.12253030472774144No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT237660.12207827710905943No Hit
TCTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCG236790.12163138616786241No Hit
ATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA223650.11488179195254203No Hit
TACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC219240.11261651718164684No Hit
GGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGAG218100.1120309359483542No Hit
AGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT215760.1108289534168588Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATA212770.10929308684883689No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA206830.10624190042273314No Hit
ACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAGAG201630.10357082813052113No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT197990.10170107752597271TruSeq Adapter, Index 7 (100% over 43bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC196540.10095625929064436Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)