FastQCFastQC Report
Sat 16 Sept 2023
SRR15906254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4409347
Total Bases661.4 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA44256110.03688301238256Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG1549623.514397936928076No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA1282412.908389836408883No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC473701.0743087355111767Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA397280.9009950906562808No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA327800.7434207378099297No Hit
ATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT265550.6022433707304052No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT257300.5835331172620345No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC233850.5303506392216354Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG230520.5227985005489475No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC228470.5181492860507463No Hit
TTGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGA201370.45668893829403767No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG199060.45145006732289383No Hit
ATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC189360.4294513450631125No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA173040.39243906183840827No Hit
ATCGGTCCGGAGTTGATGGGTTACCAGACTGTAGGCACCATCAATAGATC172090.3902845478026565No Hit
ACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG163130.36996407858125024Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAG155760.3532495854828391No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGAT154110.3495075347891649No Hit
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT150830.34206879159204295Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGTTCGTGGCGGCTAGCGTTTAGCTGTAGGCACCATCAATAGATCGG142580.32335853812367227No Hit
ATCGGTCCGGAGTTGATGGGTTACCAGATCTGTAGGCACCATCAATAGAT139140.31555692940473956No Hit
ACCGGTCCGGAGTTTGTGGGTTACCAAAACTGTAGGCACCATCAATAGAT109840.249107180723132No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT108670.24645372659489037TruSeq Adapter, Index 1 (100% over 43bp)
ATAGGTCCGGAGTCGGTGGGTTATCTGACTGTAGGCACCATCAATAGATC102910.23339056780970063No Hit
AGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAAG100430.22776615222163282No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA100420.22774347312651966Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA100330.22753936127050106No Hit
ATCGGTCCGAAGTTGATGGGTTACCAATTCTGTAGGCACCATCAATAGAT98120.222527281250489No Hit
TTTCGGTCCGGAGTCAATGGGTTATCTTCTGTAGGCACCATCAATAGATC97420.22093974459256668No Hit
TCGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGA94230.2137051132514633No Hit
TTTCGGTCCGGAGTCAATGGGTTATCTTTCCTGTAGGCACCATCAATAGA94060.2133195686345393No Hit
ATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT92480.2097362716066574No Hit
ATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG91610.20776319033181104No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT90830.20599422091298328No Hit
AGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT89160.20220681202908278Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA87420.19826064947939004No Hit
AGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA85880.19476806883196085Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC78200.17735052378504118No Hit
GTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA78000.17689694188277766No Hit
AATGAGTTGAATACAAATGATTCTTCCTGTAGGCACCATCAATAGATCGG77580.17594441988802423No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG77290.1752867261297421TruSeq Adapter, Index 1 (100% over 49bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA76140.1726786301917268No Hit
TTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAA75810.17193022005299197No Hit
TTTCGGTCCGGAGTCAATGGGTTATCTTTCTGTAGGCACCATCAATAGAT75050.17020660882439057No Hit
AGGGTTGTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA73220.16605633441867923Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC72700.16487702147279404No Hit
AGTAGAGCAGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC71890.16304001476862676No Hit
ACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT69880.15848151665087823Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA68990.15646307718580552No Hit
AAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAA68100.1544446377207328No Hit
TCGAGTACAGACCGCGAAAGCGTGGCCTATCGCTGTAGGCACCATCAATA68100.1544446377207328No Hit
ACCGGTCCGGAGTTTGTGGGTTACCAAACTGTAGGCACCATCAATAGATC66510.15083866159773773No Hit
GTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAA65480.14850271480108054No Hit
ACACGAGAGCTTATGCCCTATGTATGGCCTGTAGGCACCATCAATAGATC65160.14777698375745887No Hit
TGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGAA64480.14623480528976285No Hit
TTGGGTTCGTGGCGGCTAGCGTTTCTGTAGGCACCATCAATAGATCGGAA63560.1441483285393506No Hit
TCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG63020.1429236574032391Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGGTCCGGAGTCAATGGGTTATCTTCTGTAGGCACCATCAATAGATCG60630.13750335367118985No Hit
CTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA60240.13661886896177597No Hit
TTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATA57830.1311532070395004No Hit
TGCGGTCCGGATCGTATGGGTTATCAATCTGTAGGCACCATCAATAGATC57630.13069962513723687No Hit
ATCGGTCCGGAGTTGATGGGTTACCCTGTAGGCACCATCAATAGATCGGA57370.13010996866429428No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA56790.12879458114773004No Hit
TAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT56710.12861314838682464TruSeq Adapter, Index 1 (100% over 35bp)
ATCTCTATCTCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC55910.1267988207777705No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAAT53820.12205888989911659No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAATA53600.1215599498066267No Hit
AGTAGAGCAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG53600.1215599498066267Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG53380.1210610097141368No Hit
TTGCGGTCCGGATCGTATGGGTTATCAATCTGTAGGCACCATCAATAGAT52480.11901989115395092No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG52390.11881577929793233No Hit
TTCGGTCCGGAGTCAATGGGTTATCTTTCCTGTAGGCACCATCAATAGAT52280.11856630925168737No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCAT52070.11809004825431067No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC50680.11493765403357913TruSeq Adapter, Index 1 (100% over 50bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT50630.11482425855801325No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC50240.11393977384859937Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTGATTGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA50230.11391709475348617Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCTTTGATTGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT48490.10997093220379345No Hit
AATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGT47360.1074081944560045TruSeq Adapter, Index 1 (100% over 46bp)
ATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA46850.10625156060523248No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA45730.10371150195255668No Hit
TCGTGGTCCGGAGTCGGTGGGTTACCTTTGCTGTAGGCACCATCAATAGA45440.10305380819427457No Hit
TTCAGGGTTGTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC45290.10271362176757692No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG45120.10232807715065291TruSeq Adapter, Index 1 (100% over 50bp)
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAA44820.10164770429725763No Hit
TTTACTGTCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA44450.10080857777807009Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)