FastQCFastQC Report
Sat 16 Sept 2023
SRR15906255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34886447
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA17386714.983800729263144No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA13305143.813842091744109No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA8947042.5646177152978633No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT8573552.457558948321679No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG5515781.5810667105194174No Hit
TTGGGTTCGTGGCGGCTAGCGTTTCTGTAGGCACCATCAATAGATCGGAA2912510.8348542916967153No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCAT1844000.5285720268389612No Hit
TTGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGA1690610.4846036628493581No Hit
TTGGGTTCGTGGCGGCTAGCGTTTAGCTGTAGGCACCATCAATAGATCGG1677680.4808973524876294No Hit
ATGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA1528690.4381902232692254No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1516210.4346129028272785Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG1401120.4016230142324324No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC1395050.39988308353670987No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG1385480.39713989790935145No Hit
TTGGGTTCGTGGCGGCTAGCCTGTAGGCACCATCAATAGATCGGAAGAGC1377920.39497286725701813No Hit
ATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1316050.37723818650835955No Hit
TTACCCCAACGCAAGGCGGGGTCTGTAGGCACCATCAATAGATCGGAAGA1257240.36038063721421676No Hit
ATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1256880.36027744527839134No Hit
TTTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG1117200.32023897417813857No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC1045070.2995633232584562No Hit
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAG1036160.2970093228467777No Hit
ACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT910830.26108419696623164Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG887530.25440538556419917Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA870620.2495582310230675No Hit
ATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC861560.24696123397146175No Hit
TCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG847020.2427934263411806Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA843580.2418073700655157No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA831120.23823578250889238No Hit
TTGGGTTCGTGGCGGCTAGCGTTCTGTAGGCACCATCAATAGATCGGAAG785470.22515047175770006No Hit
AGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT779720.22350226722715558Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG774410.22198018617373105No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT764350.21909654485594363No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGAT757470.2171244323046139No Hit
CCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATAG748780.21463349363149536No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTCTGTAGGCACCATC737060.2112740228318464No Hit
GTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA658620.18878964659255784No Hit
TGTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG652880.18714430850467517No Hit
TCGAGTACAGACCGCGAAAGCGTGGCCTATCGCTGTAGGCACCATCAATA633330.18154041310082394No Hit
AGTAGAGCAGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC610510.1749991909465587No Hit
AGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA595230.1706192665593031Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAA589680.16902839088199495No Hit
TGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGAA588880.16879907546904963No Hit
TCGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGA587230.1683261124298499No Hit
AGGGTTGTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA555690.15928535227448068Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGAAGAGCACACG543110.15567936740591554No Hit
ATCGGTCCGGAGTTGATGGGTTACCAGATCTGTAGGCACCATCAATAGAT539100.15452992389852713No Hit
TCGTGGTCCGGAGTCGGTGGGTTACCTTTGCTGTAGGCACCATCAATAGA538930.15448119437327623No Hit
TCAATGAGTTGAATACAAATGATTCTTCCTGTAGGCACCATCAATAGATC532780.1527183321362591No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAG522130.14966557070142456No Hit
ACCGGTCCGGAGTTTGTGGGTTACCAAAACTGTAGGCACCATCAATAGAT509130.1459391952410631No Hit
AATGAGTTGAATACAAATGATTCTTCCTGTAGGCACCATCAATAGATCGG498570.1429122317901849No Hit
ATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA476520.13659172572087952No Hit
ACACGAGAGCTTATGCCCTATGTATGGCCTGTAGGCACCATCAATAGATC475630.13633661232397784No Hit
TTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATA473590.1357518580209673No Hit
ATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC467590.1340319924238774No Hit
TAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA464490.13314339519871426Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGTCCGGAGTTGATGGGTTACCAGACTGTAGGCACCATCAATAGATC459360.13167291011320242No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA448590.12858575136642605No Hit
TTGCGGTCCGGATCGTACTGTAGGCACCATCAATAGATCGGAAGAGCACA444330.12736464679249224No Hit
ACAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG438220.12561325032612236Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGAGGCATCTCTATCTCGTGGCTGTAGGCACCATCAATAGATCGGAA433830.1243548819975849No Hit
ACGCCGACGGCGTTGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC427160.1224429647421533No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG415590.11912649058243162No Hit
ACCCCAACGCAAGGCGGGGTCTGTAGGCACCATCAATAGATCGGAAGAGC410940.11779359474468697No Hit
TGCGGTCCGGATCGTACTGTAGGCACCATCAATAGATCGGAAGAGCACAC409530.11738942632937083No Hit
TCGTGGTCCGGAGTCGGTGGGTTACCCTGTAGGCACCATCAATAGATCGG406260.11645209957895683No Hit
TTGCGGTCCGGATCGTATGGGTTATCAATCTGTAGGCACCATCAATAGAT405330.1161855204114079No Hit
TCTTGGTCAAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC403520.11566669428961912No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT399820.11460611050474702No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACAGGGTTGTTCTGTAGGCACCATCA397130.11383503742871837No Hit
TTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATC392100.11239321676982468No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG379430.10876143391730318No Hit
TACCCCAACGCAAGGCGGGGTCTGTAGGCACCATCAATAGATCGGAAGAG378910.10861237889888872No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA358930.10288522646057936No Hit
TCTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG357670.1025240546851905No Hit
ATCTCTATCTCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC351010.10061500387242071No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)