FastQCFastQC Report
Sat 16 Sept 2023
SRR15906256_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906256_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29678543
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA5609041.8899310522083244No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA5350391.8027805475491165No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC4543481.5308972546260105No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA3684701.2415366886440484No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT3308561.1147986611067802No Hit
AAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAA2770320.9334420493620593No Hit
TTGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGA2629980.8861553614677109No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAAT1865320.6285079425900388No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA1610610.5426849963625237No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1491420.5025246690850019No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGCTGTAGGCACCATCAAT1158220.39025500679059616No Hit
TTGGGTTCGTGGCGGCTAGCGTTTCTGTAGGCACCATCAATAGATCGGAA1114610.37556088922559305No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG1092520.36811780147024065No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGAT1058180.3565471526011233No Hit
AAAAAGAAACTAAAAAGGATTCCCTTAGTAACGCTGTAGGCACCATCAAT1051510.3542997376926489No Hit
AGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAAG937540.31589825686523765No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGAACTGTAGGCACCATCAATAGA930750.3136104087050365No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCAT930740.31360703926739264No Hit
TACTCTGGTGGAAGTCCGTATCGGTTCTGTAGGCACCATCAATAGATCGG922130.31070595345600355No Hit
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAA899310.3030168967526472No Hit
AAAAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGG849070.2860888420297452No Hit
AAAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGA743850.25063561914073745No Hit
TCGAGTACAGACCGCGAAAGCGTGGCCTATCGCTGTAGGCACCATCAATA729120.2456724374912879No Hit
TTTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG714390.2407092558418383No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA677390.2282423365594463No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA665460.22422259745028586No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG664420.22387217593532135No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG662330.2231679634677484No Hit
TGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAG661000.22271982826111109No Hit
AAGTGCAGCCTAAAGTGGGTGATCTGTAGGCACCATCAATAGATCGGAAG656850.22132151163889685No Hit
TTGACGACAGTGAAATACCACCACTCTGTAGGCACCATCAATAGATCGGA641340.21609551385322387No Hit
TTGGGTTCGTGGCGGCTAGCGTTTAGCTGTAGGCACCATCAATAGATCGG637520.214808388673258No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC621670.20946783000769276No Hit
TACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGAT610960.20585916229108686No Hit
TCGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA587870.1980791307713455No Hit
TTGCGGTCCGGATCGTATGGGTTATCAATCTGTAGGCACCATCAATAGAT543840.183243496825299No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA540750.18220234059333706No Hit
ATGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA538300.1813768283705841No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG535580.1804603413314461No Hit
TTGCGGTCCGGATCGTATGGGTTATCCTGTAGGCACCATCAATAGATCGG528370.1780309767902016No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG508190.1712314516248321No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA492430.1659212178980619No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTCTGTAGGCACCATC463770.15626440961067395No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAATA456580.15384178394471723No Hit
AGGCAGTTATATTCGGTAAAGCTCTGTAGGCACCATCAATAGATCGGAAG445590.15013877197408243No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGACTGTAGGCACCATCAATAGAT442370.14905381305274992No Hit
TAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAA431040.14523624020222287No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA419340.14129399815887186No Hit
TACTCTGGTGGAAGTCCGTATCGGTTCTGACCTGTAGGCACCATCAATAG413640.13937341870185474No Hit
TACTCTGGTGGAAGTCCGTATCGGCTGTAGGCACCATCAATAGATCGGAA411170.13854116760381396No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA407390.13726752017442367No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC382180.12877316787417764No Hit
TAGGCAGTTATATTCGGTAAAGCTCTGTAGGCACCATCAATAGATCGGAA381700.12861143486727095No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA379030.1277117950163524No Hit
TGGCAGTTATATTCGGTAAAGCTCTGTAGGCACCATCAATAGATCGGAAG373670.1259057764392275No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT372360.12546438010787794No Hit
TTGCCCTGCCGAGGAGCTATCCGGAAACGAAAGTCTGTAGGCACCATCAA371840.12528916935039566No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACCTGTAGGCACCATCAATA364140.12269470236460059No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC353730.11918711777731136No Hit
TTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATA350670.1181560698582811No Hit
TTTCGGTCCGGAGTCAATGGGTTATCTTTCCTGTAGGCACCATCAATAGA347700.11715534687804588No Hit
CTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA347460.1170744803745925No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTCTGTAGGCACCATCAATAGATC340420.11470239627329414No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCGCCTGTAGGCACCATCAATAGAT337160.1136039596013861No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT333580.11239770092487357No Hit
TTACCCCAACGCAAGGCGGGGTGCCTGTAGGCACCATCAATAGATCGGAA330900.11149469163631112No Hit
TACTCTGGTGGAAGTCCGTATCGGTCTGTAGGCACCATCAATAGATCGGA330430.1113363280670483No Hit
TACTCTGGTGGAAGTCCGTATCGGTTCCTGTAGGCACCATCAATAGATCG327620.11038951608911529No Hit
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT325950.10982682000258571Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTGTAGGCACCATCAATAGATC322690.10872838333067765No Hit
TCTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG322550.10868121120366321No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGAACCTGTAGGCACCATCAATAG319880.10778157135274463No Hit
AGTGCAGCCTAAAGTGGGTGATCTGTAGGCACCATCAATAGATCGGAAGA316330.10658542098916379No Hit
TCTCTGGTGGAAGTCCGTATCGGTTCTGTAGGCACCATCAATAGATCGGA316230.10655172661272488No Hit
TAAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGA312680.10535557624914403No Hit
TTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGATCGGA310880.10474907747324388No Hit
TCTGGATCTCAGGCAGTTATATTCGGTAAAGCTCTGTAGGCACCATCAAT308650.10399769287865648No Hit
TTGGGTTCGTGGCGGCTAGCCTGTAGGCACCATCAATAGATCGGAAGAGC303760.1023500378707944No Hit
TTAGCTTCAGCGATGGATCGGTTGCATCGAGTCTGTAGGCACCATCAATA301570.10161213102678256No Hit
TCAACCTGAACTCAGTCGTGATTACCCGCTGAACTGTAGGCACCATCAAT300820.10135942320349083No Hit
TTGCCCTGCCGAGGAGCTATCCGGAAACGAAAGCTGTAGGCACCATCAAT298290.10050695547958671No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA297720.10031489753388499No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)