FastQCFastQC Report
Sat 16 Sept 2023
SRR15906257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20724491
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA6097622.9422290757345984No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC3395411.638356280981762No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA3270441.5780556444064175No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA2100671.013617173999593No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAAT1849670.8925044286974286No Hit
TTGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGA1817560.8770106826749086No Hit
AAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAA1773880.8559341698669463No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT1771880.8549691280717099No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1284370.6197353652738685No Hit
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAA1039120.5013971151330086No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG927530.4475526081677953No Hit
TTGGGTTCGTGGCGGCTAGCGTTTCTGTAGGCACCATCAATAGATCGGAA863560.41668574634716No Hit
TTGACGACAGTGAAATACCACCACTCTGTAGGCACCATCAATAGATCGGA829290.40014975518578483No Hit
TGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAG747900.3608773793286407No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGAT712220.3436610337016238No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA705820.34057289995686746No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCAT683870.32998156625414826No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGCTGTAGGCACCATCAAT673920.3251804833228474No Hit
TCGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA599420.28923267645029255No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG594880.287042031575106No Hit
TCGAGTACAGACCGCGAAAGCGTGGCCTATCGCTGTAGGCACCATCAATA590210.2847886589832291No Hit
AGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAAG571140.2755869854656503No Hit
ATGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA557820.26915980710937604No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA548620.2647206148512887No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG543890.2624382910055547No Hit
TACTCTGGTGGAAGTCCGTATCGGTTCTGTAGGCACCATCAATAGATCGG541260.2611692610448189No Hit
AAAAAGAAACTAAAAAGGATTCCCTTAGTAACGCTGTAGGCACCATCAAT537280.2592488278722985No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG530740.2560931412018756No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC524950.2532993452046663No Hit
AAAAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGG506900.24458984300265804No Hit
TACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGAT476320.22983435395349397No Hit
TTTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG474620.22901406842754304No Hit
AAAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGA465200.22446872157197975No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA451460.2178388844387059No Hit
TTGCGGTCCGGATCGTATGGGTTATCCTGTAGGCACCATCAATAGATCGG422520.2038747296616356No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG413880.1997057491062145No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA408510.19711461188600482No Hit
AAGTGCAGCCTAAAGTGGGTGATCTGTAGGCACCATCAATAGATCGGAAG405370.19559949626748374No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGAACTGTAGGCACCATCAATAGA380900.18379220990276673No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA377490.18214681364188873No Hit
TGGCAGTTATATTCGGTAAAGCTCTGTAGGCACCATCAATAGATCGGAAG371520.17926616388310818No Hit
TTGCGGTCCGGATCGTATGGGTTATCAATCTGTAGGCACCATCAATAGAT366120.17666055103596995No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA349320.16855419995598445No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT349030.16841426889567518No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAATA339200.1636710884720884No Hit
TAGGCAGTTATATTCGGTAAAGCTCTGTAGGCACCATCAATAGATCGGAA320430.15461417122379506No Hit
TAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAA317820.1533547916810116No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTCTGTAGGCACCATC317390.1531473076950358No Hit
AGGCAGTTATATTCGGTAAAGCTCTGTAGGCACCATCAATAGATCGGAAG301550.14550417667676374No Hit
TTTCGGTCCGGAGTCAATGGGTTATCTTTCCTGTAGGCACCATCAATAGA300500.14499752973426464No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC293890.14180806660100845No Hit
TTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATA292720.14124351715079517No Hit
TGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCA284960.13749915498527804No Hit
TTTCGGTCCGGAGTCAATGGGTTATCTTTCTGTAGGCACCATCAATAGAT277420.13386094741723692No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTCTGTAGGCACCATCAATAGATC277030.13367276426716584No Hit
TTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAA274500.13245198639619182No Hit
TTGGGTTCGTGGCGGCTAGCGTTTAGCTGTAGGCACCATCAATAGATCGG270800.13066665907500455No Hit
TTGGGTTCGTGGCGGCTAGCCTGTAGGCACCATCAATAGATCGGAAGAGC266720.12869797381272235No Hit
TCTGGATCTCAGGCAGTTATATTCGGTAAAGCTCTGTAGGCACCATCAAT258770.12486193267665778No Hit
TACTCTGGTGGAAGTCCGTATCGGCTGTAGGCACCATCAATAGATCGGAA258630.12479437975099124No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT245540.11847818120116918No Hit
AGTGCAGCCTAAAGTGGGTGATCTGTAGGCACCATCAATAGATCGGAAGA244650.118048737602289No Hit
TAAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGA240340.11596907253355462No Hit
TCTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG239570.11559753144238863No Hit
TCTCTGGTGGAAGTCCGTATCGGTTCTGTAGGCACCATCAATAGATCGGA238570.11511501054477043No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA232810.11233569017448969No Hit
TACTCTGGTGGAAGTCCGTATCGGTTCTGACCTGTAGGCACCATCAATAG232290.11208477930772824No Hit
TCGGCTTGTCGGGCAACATTAGTCTGTAGGCACCATCAATAGATCGGAAG231910.11190142136663334No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC230660.11129827024461059No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA229630.11080127372006386No Hit
TTAGCTTCAGCGATGGATCGGTTGCATCGAGTCTGTAGGCACCATCAATA224250.10820531129087803No Hit
TATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT223040.10762146100476001No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA222620.10741880222776039No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG220810.10654543940307147No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA220740.10651166294023819No Hit
TTCGGCTTGTCGGGCAACATTAGTCTGTAGGCACCATCAATAGATCGGAA220700.10649236210433348No Hit
GTGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA220100.10620284956576256No Hit
TTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGATCGGA213020.10278660161062582No Hit
CTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA208040.10038364754048724No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)