FastQCFastQC Report
Sat 16 Sept 2023
SRR15906258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21911532
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA3728411.701574312558337No Hit
TTGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA3231221.474666399410137No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC2624941.1979719172534353No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA2015240.9197166131514674No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT1903610.8687708372011596No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAAT1568790.7159654559982387No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA1567000.7151485345707457No Hit
AAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAA1312630.5990589795364377No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA1196520.5460686181139685No Hit
TTGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGA1177430.537356310822995No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA981680.4480197915873705No Hit
TTGGGTTCGTGGCGGCTAGCGTTTCTGTAGGCACCATCAATAGATCGGAA938210.4281809231778043No Hit
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAA855360.39036978336339057No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG817670.3731687953174611No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGCTGTAGGCACCATCAAT793500.362138074142876No Hit
TACTCTGGTGGAAGTCCGTATCGGTTCTGTAGGCACCATCAATAGATCGG732930.33449509600697935No Hit
TTGACGACAGTGAAATACCACCACTCTGTAGGCACCATCAATAGATCGGA731640.3339063649223614No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG716640.3270606546361067No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA708720.32344611960496417No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG706270.3223279869248759No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC680030.3103525577307876No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGAT678410.3096132210198721No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG628970.2870497599163765No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG624780.28513752484308263No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGAACTGTAGGCACCATCAATAGA566640.2586035517735592No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAATA524520.23938079728975595No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA514520.23481699043225276No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCAT502190.22918981657695137No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG481170.21959669456247968No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT480870.21945978035675462No Hit
AAAAAGAAACTAAAAAGGATTCCCTTAGTAACGCTGTAGGCACCATCAAT473390.21604605282734224No Hit
TGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAG472170.21548926839072688No Hit
ATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC449620.2051978839270572No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC447610.20428055874869908No Hit
AGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAAG434380.1982426422762224No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT380290.1735570109839878No Hit
TTTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG373160.17030301669458806No Hit
TTGGGTTCGTGGCGGCTAGCGTTTAGCTGTAGGCACCATCAATAGATCGG370500.1690890440704922No Hit
AAAAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGG366980.16748258405665106No Hit
TACTCTGGTGGAAGTCCGTATCGGCTGTAGGCACCATCAATAGATCGGAA362630.1654973280736372No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC359340.16399583561751868No Hit
ATGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA337880.1542019061013169No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA335340.15304269915951108No Hit
TCGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA334140.15249504233661068No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA321050.14652101916013904No Hit
GCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAA286210.13062071606859804No Hit
AAAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGA285800.1304335999874404No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGACTGTAGGCACCATCAATAGAT285720.13039708953258036No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA283950.12958929571880232No Hit
TACTCTGGTGGAAGTCCGTATCGGTTCTGACCTGTAGGCACCATCAATAG281920.1286628429267292No Hit
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT274810.12541797625104442Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCAGTTATATTCGGTAAAGCTCTGTAGGCACCATCAATAGATCGGAAG272930.12455998056183383No Hit
TACTCTGGTGGAAGTCCGTATCGGTCTGTAGGCACCATCAATAGATCGGA270630.1235103049846081No Hit
TTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGATCGGA264900.1208952436552588No Hit
TACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGAT264850.12087242462097128No Hit
TTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATA262230.11967670722430544No Hit
TTTCGGTCCGGAGTCAATGGGTTATCTTTCCTGTAGGCACCATCAATAGA257830.11766863220700405No Hit
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA257600.11756366464928149No Hit
TACTCTGGTGGAAGTCCGTATCGGTTCCTGTAGGCACCATCAATAGATCG253660.11576552474742523No Hit
TCGTGAATGCGAGGTCCAAGTGGGCCTGTAGGCACCATCAATAGATCGGA249650.11393543819756646No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA248980.11362966313811376No Hit
TTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAA247070.11275797602833064No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT244510.11158964147280984No Hit
TCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA243260.11101916561562193No Hit
AAGTGCAGCCTAAAGTGGGTGATCTGTAGGCACCATCAATAGATCGGAAG243210.11099634658133443No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA242820.1108183581138918No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG241820.1103619774281415No Hit
TATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA241270.11011096805097881No Hit
TTTCGGTCCGGAGTCAATGGGTTATCTTTCTGTAGGCACCATCAATAGAT235970.10769215041650214No Hit
TTGCGGTCCGGATCGTATGGGTTATCCTGTAGGCACCATCAATAGATCGG235710.10757349143820706No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA235110.10729966302675686No Hit
ACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT234520.10703039842216418No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCGCCTGTAGGCACCATCAATAGAT233300.1064736139855488No Hit
TCTCTGGTGGAAGTCCGTATCGGTTCTGTAGGCACCATCAATAGATCGGA232220.10598072284493845No Hit
CTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA228520.10429211430766229No Hit
TTAGCTTCAGCGATGGATCGGTTGCATCGAGTCTGTAGGCACCATCAATA228450.10426016765965976No Hit
TTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATCGGAAGAGCACAC226290.10327438537843908No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA219770.10029878330734701No Hit
TTGCGGTCCGGATCGTATGGGTTATCAATCTGTAGGCACCATCAATAGAT219610.10022576239762697No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)