FastQCFastQC Report
Sat 16 Sept 2023
SRR15906259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22054014
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA6411972.907393638183054No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA5348182.4250370023343595No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA5139222.330287810645264No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC3112151.411149008974058No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA2260611.0250333567395031No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT2025630.9184858593088768No Hit
TTGACGACAGTGAAATACCACCACTCTGTAGGCACCATCAATAGATCGGA1727200.7831680890381224No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG1515810.6873170571125964No Hit
TTGGGTTCGTGGCGGCTAGCGTTTCTGTAGGCACCATCAATAGATCGGAA1329010.6026159228882325No Hit
TTGGGTTCGTGGCGGCTAGCGTTTAGCTGTAGGCACCATCAATAGATCGG1275340.5782802169255901No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA1195070.5418832145477009No Hit
TCTTGGTCAAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1177320.5338347930676021No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC1048430.47539191731718317No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT1045710.47415858174389475No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT1021150.46302228700861436No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCAT994880.45111062321806816No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA928270.42090750463838467No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA837800.3798854938606641No Hit
AAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAA813940.369066601662627No Hit
TTTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG811380.36790581524070853No Hit
TTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATA785290.3560757692454535No Hit
TTGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGA767280.34790945539437856No Hit
TTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAA684260.3102655144773192No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG665530.3017727294450797No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG663450.3008295904772709No Hit
ATGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA656790.29780973205149863No Hit
TCGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA647750.29371070499909907No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG621560.2818353157842377No Hit
TTGGGTTCGTGGCGGCTAGCCTGTAGGCACCATCAATAGATCGGAAGAGC611750.27738714594087044No Hit
AGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT606800.275142656570364Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAA581290.26357560124882484No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGAT575930.261145204677933No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA552670.2505983717975331No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA548170.24855792691525452No Hit
TCAAGGAGAGGGCAAGTCTGGTGCTGTAGGCACCATCAATAGATCGGAAG543680.24652201635493656No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAAT535190.242672377010371No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA531670.2410762956802331No Hit
TGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAG502050.22764563403287946No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC498710.2261311704980327No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG455680.2066199831014889No Hit
CGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGA450280.20417144924275463No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG445970.2022171564777278No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG426900.1935702044988273No Hit
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT396110.17960902718208124Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTACCCCAACGCAAGGCGGGGTCTGTAGGCACCATCAATAGATCGGAAGA395760.17945032591345955No Hit
TGTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG375430.17023204936752104No Hit
AAAAAGAAACTAAAAAGGATTCCCTTAGTAACGCTGTAGGCACCATCAAT363420.16478632869281756No Hit
GTGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA362720.1644689261555742No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGCTGTAGGCACCATCAAT347030.15735457499936292No Hit
TTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGG346740.15722307966250498No Hit
ATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC337310.15294721405364123No Hit
TCAAGGAGAGGGCAAGTCTGGTGCCTGTAGGCACCATCAATAGATCGGAA325560.14761938574991385No Hit
TGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGA316900.1436926629320177No Hit
TACTCTGGTGGAAGTCCGTATCGGTTCTGTAGGCACCATCAATAGATCGG311830.14139376169798387No Hit
TACTCTGGTGGAAGTCCGTATCGGCTGTAGGCACCATCAATAGATCGGAA309270.14023297527606537No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT305330.1384464524235815No Hit
AGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAAG294460.1335176444523886No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT288830.13096482118856004No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG286240.12979043180075972No Hit
TTGACGACAGTGAAATACCACCACTGTAGGCACCATCAATAGATCGGAAG276320.1252923844158256No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGAT275900.12510194289347962No Hit
TTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGG274990.12468931959506328No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA272410.12351946452922356No Hit
CTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA271650.12317485606021651No Hit
TTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGATCGGA265460.12036811076659333No Hit
TAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA263130.11931161374976909Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGG262650.11909396629565937No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA261830.1187221518948886No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA257360.1166953099784919No Hit
TTTCGGTCCGGAGTCAATGGGTTATCTTTCCTGTAGGCACCATCAATAGA253270.11484077229659871No Hit
TCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG252380.1144372176421036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA244900.11104554481556055No Hit
TGGCAGTTATATTCGGTAAAGCTCTGTAGGCACCATCAATAGATCGGAAG243780.11053770075597122No Hit
TCTTGGTCAAGGTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC242310.10987115542776021No Hit
TCAAGGAGAGGGCAAGTCTGGTGCCCTGTAGGCACCATCAATAGATCGGA241000.10927715925091912No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAAT240550.10907311476269128No Hit
TCTCCAGTTGGTACGTCTGGTTCTGTAGGCACCATCAATAGATCGGAAGA240110.10887360459642403No Hit
AAGGAGAGGGCAAGTCTGGTGCTGTAGGCACCATCAATAGATCGGAAGAG239220.10847004994192894No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGA233270.10577212837536061No Hit
TCAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGAT231100.10478818050990628No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA231050.10476550890010318No Hit
TCGACGACAGTGAAATACCACCACTCTGTAGGCACCATCAATAGATCGGA229880.10423499323071075No Hit
AAAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGA228370.10355031061465728No Hit
AGTAGAGCAGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC224420.10175925344021275No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)